Modulation of Immune Biomarkers by Biofield Energy Healing Based Herbomineral Formulation in Male Sprague Dawley Rat: Potential Role of Energy of Consciousness

Journal: Advances in Bioscience and Bioengineering PDF  

Published: 11-Dec-17 Volume: 5 Issue: 6

DOI:10.11648/j.abb.20170506.12 ISSN: 2330-4154 (Print)2330-4162 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Alan Joseph Balmer, Dimitrius Anagnos, Janice Patricia Kinney, Joni Marie Holling, Joy Angevin Balmer, Lauree Ann Duprey-Reed, Vaibhav Rajan Parulkar, Mayank Gangwar, Snehasis Jana

Abstract

The present study aimed to evaluate the impact of The Trivedi Effect®- Energy of Consciousness Healing Treatment based new proprietary herbomineral formulation and Energy Healing Treatment per se in male Sprague Dawley rats for immune biomarkers modulation. The test formulation was divided into two parts, one as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from seven renowned Biofield Energy Healers. Additionally, one group of animals also received Biofield Energy Treatment per se (day -15) by under similar conditions. Humoral immune response data showed an increased level of IgG by 2.89%, 6.20%, and 6.20% in the Biofield Energy Treated test formulation group (G4), Biofield Energy Treatment group per se (G6), and Biofield Energy Treated test formulation at day -15 (G7), respectively as compared with the disease control group (G2). The ratio of CD4+/CD8+ was increased by 3.80%, 12.38%, and 16.19% in the G4, G6, and G7, respectively compared with the G2. Hematology analysis suggested an increased level of TLC and neutrophil by 9.64% and 1.48%, respectively in the G4 group, while G6 group showed an increase count of TLC, lymphocytes, and monocytes by 19.79%, 2.87%, and 15.54%, respectively compared with the G2. Biochemical study showed an increased concentration of glucose by 11.78%, 18.60%, and 56.84% in the G4, G6, and G7, respectively compared with G2. Total cholesterol was significantly decreased by 10.54% and 4.20% in the G4 and G7, respectively compared with G2. In contrast, the HDL level was increased by 11.69% and 1.06% in G6 and G7, respectively while LDL was decreased by 10.52% in G4 compared with the G2. SGOT, CK-MB, total protein, albumin, and globulin levels were decreased by 4.04%, 23.54%, 1.68%, 1.54%, and 1.80%, respectively in G4, while G6 group showed decreased level of SGOT, CK-MB, and A/G ratio by 2.08%, 16.23%, and 3.40%, respectively compared with G2. However, SGOT was significantly decreased (p≤0.01) by 18.88% in G7 compared with G2. The testosterone level was decreased by 76.67%, 18.43%, and 44.06% in G4, G6, and G7, respectively compared with G2. Antioxidant profile showed a decreased level of LPO by 59.63% in G7, while SOD and CAT levels were significantly altered in tested groups compared with G2. Biofield Energy may also be used for autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.

Immunopotentiating Impact of The Trivedi Effect®-Biofield Energy Healing on Herbomineral Formulation After Oral Administration in Male Sprague Dawley Rats

Journal: American Journal of Bioscience and Bioengineering PDF  

Published: 11-Dec-17 Volume: 5 Issue: 6

DOI:10.11648/j.bio.20170506.13 ISSN: 2328-5885 (Print)2328-5893 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ariadne Esmene Afaganis, Barbara Marie Bader, Brian A. Weekes, Daphne Luisa Dumas, Denise Marie Fiedler, Dennille Mellesia Smith, Desi Pano, Donna Felice Galla, Donna Maria Alija, Elaine Barbara Mullins, Elaine M. Scorza, Ellia O'Donnell, Fabio Massimo Paciucci, Frances Goodman Warlick, Haddon Norman Salt, Inthirani Arul, Jacqueline Y. Andrews, James Jay McLeran, James Stephen Burnett, Jean Caroline White, Mayank Gangwar, Snehasis Jana

Abstract

A new proprietary herbomineral formulation was formulated, consisting of essential ingredients viz. herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) Treatment on the herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The experimental parameters studies were humoral immune response (primary and secondary titre), delayed type hypersensitivity reaction, animal weight parameters, feed and water intake, histopathology, hematological and serum biochemistry. Humoral immune response showed the primary and secondary antibody titre values were significantly (p≤0.05) increased by 5.33% and 112.5%, respectively in the Biofield Energy Treated test formulation (G4) group with respect to the disease control group (G2). Delayed type hypersensitivity results showed significant increase in paw edema by 88.24% (p≤0.05) and 25% at 24 and 48 hours of duration, respectively in the Biofield Energy Treated test formulation (G4) group compared with the G2 group. Hematological studies showed a significant increase in the RBC count, hemoglobin, and packed cell volume (PCV), while the platelet count was increased by 8.40% in the Biofield Energy Treated test formulation group (G4) compared with the disease control group (G2). Biochemical analysis exhibited an increased level of calcium and phosphorus by 2.34% and 7.38%, respectively while serum uric acid was significantly decreased by 18.34% along with a slight increase in the magnesium, potassium, and sodium ions in the Biofield Energy Treated test formulation (G4) compared with the G2 group. In conclusion, the Biofield Energy Treated Test Formulation would be the powerful immunomodulatory product, which was found to be safe at the tested doses. Therefore, The Trivedi Effect®-Biofield Energy Healing based herbomineral formulation can be applied to potentiate the immune system that helps to fight against many infectious diseases. Overall, experimental data suggested that the Biofield Energy Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.

Evaluation of Immunomodulatory Parameters in Female Sprague Dawley Rats after Oral Administration of the Biofield Energy Healing Treated Herbomineral Formulation

Journal: American Journal of BioScience PDF  

Published: 11-Dec-17 Volume: 5 Issue: 6

DOI:10.11648/j.ajbio.20170506.12 ISSN: 2330-0159 (Print)2330-0167 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Aileen Carol Lee, Aksana Hancharuk, Carola Marina Sand, Debra Jane Schnitzer, Rudina Thanasi, Eileen Mary Meagher, Faith Ann Pyka, Gary Richard Gerber, Johanna Catharina Stromsnas, Judith Marian Shapiro, Laura Nelson Streicher, Lorraine Marie Hachfeld, Matthew Charles Hornung, Patricia M. Rowe, Sally Jean Henderson, Sheila Maureen Benson, Shirley Theresa Holmlund, Stephen P. Salters, Mayank Gangwar, Snehasis Jana

Abstract

The use of herbomineral formulations for different chronic diseases is gaining popularity because these natural formulations have fewer side effects, a higher safety profile, and more cost effectiveness. A new proprietary herbomineral formulation composed of herbs and certain minerals was formulated, consisting of the herbal root extract ashwagandha and the minerals (zinc, magnesium, and selenium). The aim of the study was to evaluate the immunomodulatory potential of a Biofield Energy Healing (The Trivedi Effect®) Treatment on the herbomineral formulation in female Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. Immunomodulatory potential, humoral immune response, paw volume, hematological study, biochemistry, body weight, feed intake and histopathology analysis were performed. Humoral immune response data showed increased (p≤0.01) primary antibody titre by 124.7% in the Biofield Treatment test formulation (G3), while the untreated test formulation group (G4) showed decreased antibody titre compared with the disease control group (G2). The secondary antibody titre value in the G3 group was also altered compared with the G2 group. Delayed type hypersensitivity (DTH) data suggest that the Biofield Energy Treated test formulation group (G3) showed a significant decrease in paw volume by 128.57% with respect to the G2 group. Hematology and biochemistry analysis showed altered values, as increased MCHC and RBC count by 138.09% and 7.24%, respectively in the G3 group compared with the G2 and G4 group. However, the platelet count was increased by 17.70% in the G3 group compared with the G2 group. The level of blood urea nitrogen was decreased by 10.90% in the G3 level compared with the G2 group. However, the levels of magnesium and phosphorus were increased by 13.29% and 6.28%, respectively in the G3 compared with the G2 group. Further, the change in body weight, feed consumption, organ to body weight ratio data, and histopathology examination did not suggest any statistical difference, which depicts that the Biofield Energy Treated test formulations was found to be safe. These data suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases such as Rheumatoid arthritis, Alzheimer’s disease, Atherosclerosis, Dermatitis, Diverticulitis, Diabetes, etc. along with enhancing stress management and anti-aging by improving overall health.

Assessment of Consciousness Energy Healing Treatment on Immune Biomarkers After Oral Administration of Herbomineral Formulation in Sprague Dawley Male Rats

Journal: Biochemistry and Molecular Biology PDF  

Published: 11-Dec-17 Volume: 2 Issue: 6

DOI:10.11648/j.bmb.20170206.17 ISSN: 2575-5064 (Print)2575-5048 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Aileen Carol Lee, Aksana Hancharuk, Carola Marina Sand, Debra Jane Schnitzer, Rudina Thanasi, Eileen Mary Meagher, Faith Ann Pyka, Gary Richard Gerber, Johanna Catharina Stromsnas, Judith Marian Shapiro, Laura Nelson Streicher, Lorraine Marie Hachfeld, Matthew Charles Hornung, Patricia M. Rowe, Sally Jean Henderson, Sheila Maureen Benson, Shirley Theresa Holmlund, Stephen P. Salters, Mayank Gangwar, Snehasis Jana

Abstract

Herbomineral formulations have increased in recognition and popularity due to their high safety and better therapeutic benefits than other conventional methods. The aim of the present study was to evaluate the immunomodulatory effect of the Trivedi Effect®- Energy of Consciousness Healing Treatment on a new proprietary herbomineral formulation in male Sprague Dawley (SD) rats. The test formulation contained essential ingredients like the herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The immunomodulatory effect of the Biofield Energy Treated and untreated test formulations was studied in SD rats to determine any alteration in humoral immune response (IgG and IgM), cellular immune response, hematology, lipid profile, hepatic enzymes, and the testosterone. The Biofield Energy Treated test formulation group (G3) showed significantly increased levels of IgM and IgG by 46.15% and 14.92%, respectively compared with the disease control group (G2). The cellular immune response (i.e. the ratio of CD4+ and CD8+) was decreased by 76.47% in the Biofield Energy Treated test formulation compared with the G2 group. The hematology parameters such as TLC, neutrophils, lymphocytes, and monocytes were significantly increased by 18.01%, 1.34%, 12.34%, and 0.67%, respectively in the Biofield Energy Treated test formulation group compared with the G2 group. The lipid profile parameters viz. total cholesterol, triglycerides, HDL, LDL, and VLDL were significantly decreased in the Biofield Energy Treated test formulation group (G3) by 8.73%, 10.96%, 6.26%, 8.60%, and 11.01%, respectively compared with the G2 group. Among the tested hepatic parameters, results showed decreased levels of SGOT (5.27%), SGPT (17.70%), ALP (0.26%), total protein (5.8%), albumin (5.47%), globulin (9.27%), and albumin/globulin ratio (1.34%) in the Biofield Energy Treated test formulation group compared with the G2 group. However, the Biofield Energy Treated test formulation group animals exhibited an increased level of testosterone by 39.8% compared with the G2 group. The overall results demonstrated that the Biofield Energy Treated test formulation showed a better immune response compared with the untreated test formulation. These experimental data suggest that the Trivedi Effect®- Energy of Consciousness Healing Treatment based herbomineral formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.

Differential Regulation of Immune Biomarkers by the Trivedi Effect®- Energy of Consciousness Healing Treatment Based Herbomineral Formulation in Male Sprague Dawley Rats

Journal: Biochemistry and Molecular Biology PDF  

Published: 11-Dec-17 Volume: 2 Issue: 6

DOI:10.11648/j.bmb.20170206.18 ISSN: 2575-5064 (Print)2575-5048 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ariadne Esmene Afaganis, Barbara Marie Bader, Brian A. Weekes, Daphne Luisa Dumas, Denise Marie Fiedler, Dennille Mellesia Smith, Desi Pano, Donna Felice Galla, Donna Maria Alija, Elaine Barbara Mullins, Elaine M. Scorza, Ellia O'Donnell, Fabio Massimo Paciucci, Frances Goodman Warlick, Haddon Norman Salt, Inthirani Arul, Jacqueline Y. Andrews, James Jay McLeran, James Stephen Burnett, Jean Caroline White, Mayank Gangwar, Snehasis Jana

Abstract

A new proprietary herbomineral formulation was formulated, consisting of essential ingredients viz. herbal root extract of ashwagandha and minerals (zinc, magnesium, and selenium). The present study aimed to evaluate the impact of The Trivedi Effect®- Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) based herbomineral formulation in male Sprague Dawley (SD) rats for immune biomarkers modulation. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The immunomodulatory potential was studied in male SD rats using various immune biomarkers such as IgG and IgM, CD4+ and CD8+, hematology, lipid profile, hepatic enzymes, sex hormone, and antioxidant profile. The humoral immune response showed an increase IgG level by 6.10% in the Biofield Energy Treated test formulation (G4) group compared with the disease control (G2) group. Cellular immune response i.e. CD4+ and CD8+ counts were significantly improved by 51.64% and 52.09%, respectively in G4 compared with the G2 group. The lymphocytes level were increased by 38.87%, while TLC, neutrophils, eosinophils, and monocytes count were decreased by 7.38%, 19.35%, 21.14%, and 3.43%, respectively in G4 compared with the G2 group. Lipid analysis showed that triglycerides and VLDL levels were decreased by 6.02% and 5.97%, respectively while HDL level was significantly increased by 28.44% in G4 compared with the G2 group. Hepatic biomarkers viz. SGOT, CK-MB, total protein, total bilirubin, and globulin by 6.27%, 14.97%, 2.85%, 16.66%, and 5.90%, respectively in G4 compared with the G2 group. However, the testosterone level was significantly increased in G4 and untreated test formulation (G5) groups by 135.74% and 28.09%, respectively compared with the G2 group. Additionally, the antioxidant assay showed that the lipid peroxidation (LPO) level was decreased, while superoxide dismutase (SOD) and catalase levels were increased by 59.72% and 20.07%, respectively in the G4 compared with the G2 group. Overall, the study results suggested that the Biofield Energy Treated test formulation showed an improved cellular and humoral immune responses along with improved hematological and biochemical animal profile compared with the untreated test formulation. Consequently, it can be established that The Trivedi Effect®-Biofield Energy Healing has the significant capacity for immunomodulatory effect, which may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.

Evaluation of Immune Biomarkers After Oral Administration of Biofield Energy Healing Based Herbomineral Formulation in Male Sprague Dawley Rats

Journal: International Journal of Biochemistry, Biophysics & Molecular Biology PDF  

Published: 11-Dec-17 Volume: 2 Issue: 6

DOI:10.11648/j.ijbbmb.20170206.15 ISSN: 2575-5889 (Print)2575-5862 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Cathryn Dawn Nykvist, Celine Lavelle, Daniel Paul Przybylski, Dianne Heather Vincent, Dorothy Felger, Douglas Jay Konersman, Elizabeth Ann Feeney, Jay Anthony Prague, Joanne Lydia Starodub, Karan Rasdan, Karen Mie Strassman, Leonid Soboleff, Maire Anne Mayne, Mary M. Keesee, Padmanabha Narayana Pillai, Pamela Clarkson Ansley, Ronald David Schmitz, Sharyn Marie Sodomora, Sambhu Charan Mondal, Snehasis Jana

Abstract

The natural products based formulations usage have been increased world-wide in health care sector due to their high safety and better therapeutic action. A new proprietary herbomineral formulation was formulated with four ingredients; a mixture of the minerals (zinc, magnesium, and selenium) and the herbal root extract ashwagandha. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation in male Sprague Dawley (SD) rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The immunomodulatory effect of the Biofield Energy Treated and untreated test formulations was studied in SD rats to determine any alteration in humoral immune response (IgG and IgM), cellular immune response, hematological parameters, lipid profile, hepatic enzymes, and the sex hormone (testosterone). The ratio of CD4+/CD8+ was significantly increased by 51.98% and 12.99% (p≤0.05) in the Biofield Energy Treated (G3) and untreated test formulations (G4), respectively compared with the disease control group (G2). The level of IgM was significantly increased by 28.57% in the G3 group, while decreased by 14.29% in the G4 group compared with the G2 group. The eosinophil count was significantly increased by 27.27% in the G3 group, while decreased by 18.18% in the G4 group compared with the G2 group. The levels of triglycerides (TG) and very low density lipoproteins (VLDL) were reduced by 8.99% and 9.12%, respectively in the G3 group compared with the G2 group. However, the levels of TG and VLDL were reduced by 2.40% and 3.46%, respectively in the G4 group compared with the G2 group. Among the tested hepatic parameters, results showed decreased levels of serum glutamate oxaloacetate transaminase (SGOT) and creatine kinase myocardium band (CK-MB) by 42.65% (p<0.01) and 7.67%, respectively in the Biofield Energy Treated test formulation group (G3) compared to the G2 group. The level of testosterone was significantly increased by 22.02% in the G3 group compared with G2 group. The overall results demonstrated that the Biofield Energy Treated test formulation showed better immune response compared with the untreated test formulation. These data suggest that the Biofield Energy Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.

Evaluation of Immune Biomarkers After Oral Administration of the Novel Herbomineral Formulation Treated with The Trivedi Effect® – Biofield Energy Healing in Male Sprague Dawley Rats

Journal: American Journal of Clinical and Experimental Medicine PDF  

Published: 11-Dec-17 Volume: 5 Issue: 6

DOI:10.11648/j.ajcem.20170506.15 ISSN: 2330-8125 (Print)2330-8133 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Michael Peter Ellis, James Jeffery Peoples, James Joseph Meuer, Johanne Dodon, John Lawrence Griffin, John Suzuki, Joseph Michael Foty, Judy Weber, Julia Grace McCammon, Karen Brynes Allen, Kathryn Regina Sweas, Lezley Jo-Anne Wright, Lisa A. Knoll, Madeline E. Michaels, Margaret Kweya Wahl, Mark E. Stutheit, Michelle Barnard, Muriel Mae Ranger, Paromvong Sinbandhit, V. J. Kris Elig, Sambhu Charan Mondal, Snehasis Jana

Abstract

Herbomineral formulations have been used worldwide against various chronic and degenerative diseases due to its fewer side effects. A new proprietary herbomineral formulation was formulated consisted of an ashwagandha root extract and minerals (zinc, magnesium, and selenium). The present study was aimed to evaluate the impact of the Biofield Energy Treated herbomineral formulation in male Sprague Dawley (SD) rats for immune biomarkers modulation. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. Biomarkers like immunoglobulins (IgG, IgM), cluster differentiation (CD4+), CD8+, superoxide dismutase (SOD), catalase (CAT), lipid peroxidation (LPO) were monitored. The level of IgM was increased by 4.76% in the Biofield Energy Treated test formulation (G4) compared to the disease control group (G2). The levels of CD4+ and CD8+ counts were significantly (p≤0.01) increased by 222.22% and 355.36% in the G4 group compared to the G2 group. The level of lymphocyte was increased by 5% and eosinophil count was significantly decreased by 75% in the G4 group compared to the G2 group. The lipid biomarkers such as total cholesterol (TC), low density lipoprotein (LDL), and very low density lipoprotein (VLDL) were significantly lowered by 9.70%, 6.67%, and 23.54%, respectively in the G4 group compared to the G2 group. The expression of SOD was reduced by 9.96% in the G4 group compared to the G2 group. Further, LPO expression was significantly reduced by 33.38% and 16.88% in the G4 and untreated test formulation (G5) groups, respectively compared to the G2 group. Therefore, it can be concluded that the Biofield Energy Treated test formulation showed significantly improved the cellular and humoral immunity, hematological and biochemical profile compared with the untreated test formulation. As a result, it can be established that The Trivedi Effect®-Biofield Energy Healing has the significant capacity for immunomodulatory effect, which may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.

An Investigation of The Trivedi Effect®-Energy of Consciousness Healing Treatment to Modulate the Immunomodulatory Effect of Herbomineral Formulation in Male Sprague Dawley Rats

Journal: American Journal of Biomedical and Life Sciences PDF  

Published: 11-Dec-17 Volume: 5 Issue: 6

DOI:10.11648/j.ajbls.20170506.16 ISSN: 2330-8818 (Print)2330-880X (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Aileen Carol Lee, Aksana Hancharuk, Carola Marina Sand, Debra Jane Schnitzer, Rudina Thanasi, Eileen Mary Meagher, Faith Ann Pyka, Gary Richard Gerber, Johanna Catharina Stromsnas, Judith Marian Shapiro, Laura Nelson Streicher, Lorraine Marie Hachfeld, Matthew Charles Hornung, Patricia M. Rowe, Sally Jean Henderson, Sheila Maureen Benson, Shirley Theresa Holmlund, Stephen P. Salters, Mayank Gangwar, Snehasis Jana

Abstract

Herbomineral formulations are used world-wide for various therapeutic purposes. More than 80% of the world population relies on natural herbal and mineral remedies as medicines for their health care. A new proprietary herbomineral formulation was created, consisting of the herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The aim of the study was to evaluate the immunomodulatory potential of the Trivedi Effect®- Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on the herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The immunomodulatory effect of the Biofield Energy Treated and the untreated test formulations were studied to determine any alterations in the animal humoral immune response, paw volume, hematological study, serum biochemistry parameters, animal weight parameters, feed intake and histopathology analysis. Humoral immune response data of the reference standard (levamisole) showed a significant increase in secondary antibody titre value (p≤0.05), while the Biofield Treated test formulation group (G3) exhibited a significant increase in the secondary antibody titre by 115.34% compared with the disease control group (G2). A delayed type hypersensitivity (DTH) reaction showed that the paw volume was significantly decreased by 114.28% in the Biofield Energy Treated test formulation group (G3) with respect to the G2 group. The platelets count was increased by 61.55% in the Biofield Treated test formulation group (G3) compared to the G2 group. Moreover, the administration of the Biofield Treated herbomineral formulation (G3) group exhibited a decrease in the level of creatinine (9.62%), and uric acid (14.40%), while the level of potassium ion concentration was incresaed by 77.43% compared to the G2 group. Further, the change in body weight, feed consumption, organ to body weight ratio data, and histopathology examination did not suggest any statistical difference, which depicts that the Biofield Energy Treated test formulation was found to be safe. Overall, The Trivedi Effect®- Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) significantly modulates the immunological parameters of the herbomineral formulation compared with the untreated test formulation. These data suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases along with stress management and anti-aging by improving overall health.

Evaluation of Immunomodulatory Properties After Oral Administration of Biofield Energy Treated Test Formulation in Sprague Dawley Rats: Influence of the Trivedi Effect®

Journal: American Journal of Biomedical and Life Sciences PDF  

Published: 11-Dec-17 Volume: 5 Issue: 6

DOI:10.11648/j.ajbls.20170506.17 ISSN: 2330-8818 (Print)2330-880X (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, Deborah Lea Smith, Dezi Ann Koster, Elizabeth Patric, Jagdish Singh, Kathleen Starr Vagt, Krista Joanne Callas, Sambhu Charan Mondal, Snehasis Jana

Abstract

A new proprietary herbomineral formulation was formulated, consisting of an essential ingredients viz. an herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The aim of this study was to evaluate the immunomodulatory potential of Energy of Consciousness Healing (The Trivedi Effect®) Treatment on the herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from seven renowned Biofield Energy Healers. Additionally, one group of animals was also received Biofield Energy Treatment per se (day -15) by Biofield Energy Healers under similar conditions. The immunological parameters viz. humoral immune analysis, paw volume, hematological study, biochemistry, body weight, feed and water intake, and histopathology analysis were performed in this experiment. The humoral immune response data showed the secondary antibody titre was significantly increased by 112.50% and 87.50% in the Biofield Energy Treated test formulation group (G4) and untreated test formulation group (G5) compared to the disease control group (G2). The results of delayed type hypersensitivity showed significant increased the paw volume by 111.76%, 51.45%, 100%, and 64.80% in the G4, G5, Biofield Energy Treatment group per se at day -15 (G6) and Biofield Energy Treated test formulation at day -15 (G7) groups, respectively compared to the G2 group. The platelet count was significantly increased by 37.72%, 8.69%, 16.30% and 33.11% in the G4, G5, G6, and G7 groups, respectively compared to the G2 group. The level of blood urea nitrogen was significantly decreased by 14.01% in the G4 group compared to the disease control. Moreover, the concentration of uric acid was significantly reduced by 28.97% in the G7 group compared to the G2 group. Animal weight parameters suggested that there were no treatment-related changes in any group. Organ to body weight ratio, feed and water intake data described that the Biofield Energy Treated test formulation was found to be safe without any side-effect during the course of the experiment. Overall, results suggested that the Biofield Energy Treated herbomineral formulation and Biofield Energy Treatment per se can be used for autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.

Immunomodulatory Activity of Biofield Energy Healing Based Herbomineral Formulation in Sprague Dawley Rats: Evidence for Humoral and Cell-Mediated Responses

Journal: American Journal of Health Research PDF  

Published: 11-Dec-17 Volume: 5 Issue: 6

DOI:10.11648/j.ajhr.20170506.14 ISSN: 2330-8788 (Print)2330-8796 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Michael Peter Ellis, James Jeffery Peoples, James Joseph Meuer, Johanne Dodon, John Lawrence Griffin, John Suzuki, Joseph Michael Foty, Judy Weber, Julia Grace McCammon, Karen Brynes Allen, Kathryn Regina Sweas, Lezley Jo-Anne Wright, Lisa A. Knoll, Madeline E. Michaels, Margaret Kweya Wahl, Mark E. Stutheit, Michelle Barnard, Muriel Mae Ranger, Paromvong Sinbandhit, V. J. Kris Elig, Sambhu Charan Mondal, Snehasis Jana

Abstract

Herbomineral formulations are used extensively in the world-wide for the prevention and treatment of a wide range of disorders. A new proprietary herbomineral formulation was formulated consisted of an ashwagandha root extract and minerals (zinc, magnesium, and selenium). The present study was aimed to evaluate the impact of the Biofield Energy Treated herbomineral formulation in male Sprague Dawley (SD) rats for immune biomarkers modulation. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The experimental parameters studies were humoral immune response (primary and secondary titre), delayed type hypersensitivity reaction, animal weight parameters, feed and water intake, histopathology, hematological and serum biochemistry. The humoral immune response exhibited that both the primary and secondary hemagglutination (HA) antibody titre levels were significantly altered by 14.79% and 12.89%, respectively in the Biofield Energy Treated test formulation group (G4) compared to the disease control group (G2). The delayed type hypersensitivity (DTH) response was significantly (p≤0.05) increased by 55.56% in the G4 group compared to the G2 group. The hematological results revealed that the platelet count was significantly increased by 8.46% in the G4 group with respect to the G2 group. Moreover, the level of phosphorus was significantly increased by 11.16% and 16.35% in the levamisole (G3) and G4 groups, respectively compared to the G2 group. In conclusion, the Biofield Energy Treated Test Formulation would be the powerful immunomodulatory product, which was found safe at the tested doses. Therefore, The Trivedi Effect®-Biofield Energy Healing based herbomineral formulation can be applied to potentiate the immune system that helps to fight against many infectious diseases. Overall, these data suggest that the Biofield Energy Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.

Effect of Biofield Energized Vitamin D3 on Bone Health in MG-63 Cell-Line

Journal: American Journal of BioScience PDF  

Published: 07-Mar-18 Volume: 6 Issue: 1

DOI:10.11648/j.ajbio.20180601.12 ISSN: 2330-0159 (Print); 2330-0167 (Online)

Authors: Faith Ann Pyka, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Abstract

The objective of current research work was to evaluate the potential of Consciousness Energy Healing-based vitamin D3 and DMEM medium on bone health parameters such as alkaline phosphatase (ALP), collagen, and bone mineralization in human bone osteosarcoma cells (MG-63). The test items like vitamin D3 and DMEM were divided into two parts. One part of each sample was received the Biofield Energy Treatment by Faith Ann Pyka and those samples were denoted as the Biofield Energy Treated (BT) samples, while the other parts of each sample were referred as the untreated test items (UT). The MTT cell viability assay revealed that the test samples were found as safe in the tested concentrations. The level of ALP was significantly increased by 191.45% in the BT-DMEM + UT-Test item (UT-TI) group at 1 µg/mL compared to the UT-DMEM + UT-TI group. Moreover, ALP level was significantly increased by 144.89% and 226.75% in the UT-DMEM + BT-TI and BT-DMEM + BT-TI groups, respectively at 50 µg/mL compared to the untreated group. Further, collagen level was significantly increased by 98.15%, 80.54%, and 49.98% in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 10 µg/mL compared to the untreated group. Additionally, at 50 µg/mL level of collagen was significantly increased by 129.73%, 189.16%, and 94.60% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively with respect to the untreated group. Apart from this, the percent of bone mineralization was distinctly enhanced by 283.74%, 27.91%, and 118.02% in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 50 µg/mL compared to the untreated group. Moreover, the percentage of bone mineralization was significantly increased by 265.92%, 231.82%, and 158.2% in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 100 µg/mL compared to the untreated group. Altogether, the Biofield Energy Treated vitamin D3 was significantly improved the bone health parameters and it could be an alternative approach for nutraceutical supplement to combat vitamin D3 deficiency and able to fight against various bone-related disorders including rickets, low bone density, osteomalacia, bone and joint pain, bone fractures, osteoporosis, osteoma, osteogenesis imperfecta, Paget’s disease, deformed bones, chondrodystrophia fetalis, stress management and prevention, autoimmune and inflammatory diseases, and anti-aging by improving overall health.

Biofield Energy Based Vitamin D3 Stimulates In vitro Osteoblasts Differentiation in MG-63 Cell line

Journal: Journal of Diseases and Medicinal Plants PDF  

Published: 08-Mar-18 Volume: 4 Issue: 1

DOI:10.11648/j.jdmp.20180401.12 ISSN: 2469-8202 (Print); 2469-8210 (Online)

Authors: Patricia Margaret Rowe, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Abstract

In most of the people, bone loss and deterioration of bone structure results in bone disorders such as osteoporosis, arthritis, decreased bone mass, rickets, and deformed bones. Insufficient level of vitamin D3 and calcium in the body are the major causes of bone disorders. The present study aimed to explore the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. Both the test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test items received the Consciousness Energy Healing Treatment by Patricia Margaret Rowe and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of both the sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability in tested samples was more than 70% with safe and nontoxic profile on MG-63 cell line. The level of ALP was significantly increased by 230.8% (at 50 µg/mL), 231.3% (at 50 µg/mL), and 253% (at 100 µg/mL) in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated test item and DMEM group. Collagen level was significantly increased by 497.7%, 346.2%, 323%, and 331.4% at 0.1, 1, 10, and 50 µg/mL, respectively in the UT-DMEM+BT-TI group, while 116.3% and 112.8% at 0.1 and 50 µg/mL, respectively in the BT-DMEM+UT-TI group. In addition, 70.5% and 143.6% increased collagen was reported at 10 and 50 µg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated group. Bone mineralization percentage was significantly increased by 241.3% at 1 µg/mL in UT-DMEM+BT-TI group, while 187.9% and 113.7% at 0.1 and 1 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 129.8% at 1 µg/mL as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.

Biofield Energy Treated Vitamin D3: Direct Effect on Bone Health Using Human Osteoblast-Like Cells

Journal: Cell Biology PDF  

Published: 09-Jan-18 Volume: 6 Issue: 1

DOI:10.11648/j.cb.20180601.11 ISSN: 2330-0175 (Print); 2330-0183 (Online)

Authors: Sheila Maureen Benson, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Abstract

Vitamin D and calcium are one of the important constituents of good bone health. Bone disorders might be to the hormonal factors and lack of proper nutrition that leads to gradual bone loss. The current study aimed to explore the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters. Vitamin D3 and DMEM medium as test items (TI), were divided into two parts. Each sample received the Consciousness Energy Healing Treatment by Sheila Maureen Benson and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were measured. MTT data showed that cell viability was more than 70% and all the test items were found to be safe at the tested concentrations. ALP level was significantly increased by 135.9% and 134.5% at 1 and 50 µg/mL, respectively in the UT-DMEM+BT-TI, while 84.4%, 93.8%, and 50.0% at 1, 50, and 100 µg/mL, respectively in BT-DMEM+UT-TI group. In addition, BT-DMEM+BT-TI group showed increased ALP by 50.2% and 104.7% at 10 and 50 µg/mL, respectively as compared with the untreated test item and DMEM group. Collagen content was significantly increased by 463.6%, 387.9%, and 239.4% in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI group, respectively at 0.1 µg/mL as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 131.7% and 81.7% at 1 and 10µg/mL, respectively in UT-DMEM+BT-TI group, while 83.1% and 53.6% at 10 and 50µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 152.3% and 108.3% at 50 and 100µg/mL, respectively as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.

Influenced of Biofield Energy Healing Treatment on Vitamin D3 for the Assessment of Bone Health Parameters in MG-63 cells

Journal: International Journal of Biochemistry, Biophysics & Molecular Biology PDF  

Published: 23-Jan-18 Volume: 3 Issue: 1

DOI:10.11648/j.ijbbmb.20180301.11 ISSN: 2575-5889 (Print); 2575-5862 (Online)

Authors: Dianne Heather Vincent, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Abstract

The objective of this experiment was to find out the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters. The test items (viz. vitamin D3 and DMEM), were divided into two parts. One part of each test item was received the Biofield Energy Treatment by Dianne Heather Vincent and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Alkaline phosphatase (ALP), collagen, and bone mineralization activities were performed to assess bone activity in human bone osteosarcoma cells (MG-63). The test samples were found as safe in the tested concentrations by MTT assay. ALP was significantly increased by 82.87%, 30.90%, and 58.53% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item, respectively at 50 µg/mL than the UT-DMEM + UT-Test item group. Other parameter like collagen was significantly increased by 11.41% and 34.65% in the in the BT-DMEM + UT-Test item group at 1 and 10 µg/mL, respectively as compared to the untreated group. Besides, the percent of bone mineralization was distinctly increased by 148.81% and 251.39% in the UT-DMEM + BT-Test item and BT-DMEM + BT-Test item groups, respectively at 10 µg/mL compared to the untreated group. Further, percent of bone mineralization was distinctly increased by 112.36%, 124.88%, and 124.69% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL compared to the untreated group. Moreover, at 100 µg/mL the percent of bone mineralization was remarkably increased by 85.97%, 102.63%, and 45.61% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively with respect to the UT-DMEM + UT-Test item group. Overall, the Biofield Energy Treated vitamin D3 was significantly improved the bone health parameters and it could be a powerful alternative nutraceutical supplement to combat vitamin D3 deficiency and fight against various bone related problems including rickets, osteomalacia, osteoporosis, osteogenesis imperfecta, Paget’s disease, bone and joint pain, low bone density, bone fractures, osteoma, chondrodystrophia fetalis, stress management and prevention, autoimmune and inflammatory diseases, and anti-aging by improving overall health.

Impact of Biofield Energy Healing Treated Vitamin D3 on Human Osteoblast Cell Line (MG-63) for Bone Health

Journal: American Journal of Clinical and Experimental Medicine PDF  

Published: 10-Jan-18 Volume: 6 Issue: 1

DOI:10.11648/j.ajcem.20180601.11 ISSN: 2330-8125 (Print); 2330-8133 (Online)

Authors: William Dean Plikerd, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Abstract

Bone disorders are largely associated with increased morbidity, mortality, and substantial economic and health costs. Vitamin D play an important role for calcium absorption and bone mineralization, which can improve the patients’ quality of life. The current study aimed to evaluate the potential of The Trivedi Effect®- Biofield Energy Healing on vitamin D3 and DMEM as test item (TI) on bone cell differentiation using human osteoblast cell line (MG-63, Osteosarcoma). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were evaluated. The test items were treated with The Trivedi Effect® by William Dean Plikerd and divided as Biofield Energy Treated (BT) and untreated (UT) test items. Cell viability using MTT data showed that the test items were found to be safe. ALP level was significantly increased by 346.4% (at 50 µg/mL), 375.3% (at 100 µg/mL), and 343.2% (at 100 µg/mL) in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared to the untreated group. Collagen content was significantly increased by 336.2% and 237.2% in the UT-DMEM+BT-TI at 10 and 50 µg/mL, respectively while 399.3% (10 µg/mL) and 200% (0.1 µg/mL) increased collagen in the BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively. Moreover, the percent of bone mineralization was significantly increased in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups by 416.9% (10 µg/mL), 460.7% (0.1 µg/mL), and 441.7% (10 µg/mL) respectively as compared with the untreated test item and DMEM group. Thus, Biofield Energy Treated vitamin D3 and DMEM would play an important role to control the osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and/or joint pain, increased frequency of fractures, deformed bones, osteoma, chondrodystrophia fetalis, hormonal imbalance, stress, aging, bone loss and fractures.

Role of Vitamin D3 on Alkaline Phosphatase, Collagen, and Bone Mineralization: Impact of Biofield Energy Healing Treatment

Journal: American Journal of Health Research PDF  

Published: 10-Jan-18 Volume: 6 Issue: 1

DOI:10.11648/j.ajhr.20180601.12 ISSN: 2330-8788 (Print); 2330-8796 (Online)

Authors: Bonnie Patrice Hegarty-Diaz, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Abstract

The objective of this study was to assess the potential of Biofield Energy Treated vitamin D3 and DMEM medium on bone health. The test items, were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Bonnie Patrice Hegarty-Diaz and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Various parameters were performed to evaluate bone strength and integrity such as ALP, collagen, and bone mineralization in human bone osteosarcoma cells (MG-63). The test samples were found as safe in the tested concentrations by MTT assay. ALP was significantly increased by 50.77% and 50.27% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item, respectively at 10 µg/mL compared to the UT-DMEM + UT-Test item group. Further, the ALP level was significantly elevated by 137.29% and 88.08% and 107.93% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL compared to the UT-DMEM + UT-Test item group. Further, the collagen level was significantly increased by 66.15%, 88.18%, and 55.87% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item and BT-DMEM + BT-Test item, respectively at 0.1 µg/mL compared to the untreated group. Collagen was significantly increased by 39.44%, 39.44%, and 49.66% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL compared to the untreated group. Additionally, the percent of bone mineralization was distinctly increased by 13.51% and 47.21% in the UT-DMEM + BT-Test item and BT-DMEM + UT-Test item groups, respectively at 10 µg/mL compared to the untreated group. The percent of bone mineralization was distinctly increased by 11.51%, 109.53%, and 83.73% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 100 µg/mL, compared to the UT-DMEM + UT-Test item group. Overall, the Biofield Energy Treated vitamin D3 was significantly improved the bone health parameters and it could be a powerful alternative nutraceutical supplement to combat vitamin D3 deficiency and fight against various bone related problems including rickets, osteomalacia, osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, bone and joint pain, low bone density, bone fractures, deformed bones, osteoma, chondrodystrophia fetalis, stress management and prevention, autoimmune and inflammatory diseases, and anti-aging by improving overall health.

A Perspective of Bone Health Study: Impact of Biofield Energy Treated Vitamin D3

Journal: International Journal of Clinical and Experimental Medical Sciences PDF  

Published: 29-Jan-18 Volume: 4 Issue: 1

DOI:10.11648/j.ijcems.20180401.12 ISSN: 2469-8024 (Print); 2469-8032 (Online)

Authors: Cathryn Dawn Nykvist, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Abstract

Bone disorder like osteoporosis is an important risk factor for fragility fractures. The main objective of this study was to find the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health. The test items (viz. vitamin D3 and DMEM), were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Cathryn Dawn Nykvist and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Alkaline phosphatase (ALP), collagen, and bone mineralization activities were performed to assess bone health in human bone osteosarcoma cells (MG-63). The test samples were found as safe in the tested concentrations by MTT assay. ALP was significantly increased by 88.68% and 166.1% in the UT-DMEM + BT-Test item and BT-DMEM + UT-Test item, respectively at 10µg/mL than the UT-DMEM + UT-Test item group. Moreover, the ALP level was significantly elevated by 51.99% and 58.32% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item, respectively at 100µg/mL compared to the untreated group. Collagen was significantly increased by 135.60%, 149.72%, and 95.90% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 100µg/mL compared to the untreated group. Further, the collagen level was significantly increased by 83.43% in the BT-DMEM + BT-Test item group at 10µg/mL, while 43.00% and 82.81% in the UT-DMEM + BT-Test item and BT-DMEM + BT-Test item groups, respectively at 50µg/mL compared to the untreated group. Besides, the percent of bone mineralization was distinctly increased by 103.28%, 120.78%, and 84.26% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 1µg/mL compared to the untreated group. Further, percent of bone mineralization was distinctly increased by 104.41% and 81.71% in the UT-DMEM + BT-Test item and BT-DMEM + UT-Test item group at 0.1µg/mL, respectively compared to the untreated group. Overall, the Biofield Energy Treated vitamin D3 was significantly improved the bone health parameters and it could be a powerful alternative nutraceutical supplement to combat vitamin D3 deficiency and fight against various bone related problems including rickets, osteomalacia, osteoporosis, osteogenesis imperfecta, Paget’s disease, bone and joint pain, low bone density, bone fractures, osteoma, chondrodystrophia fetalis, stress management and prevention, autoimmune and inflammatory diseases, and anti-aging by improving overall health.

Evaluation of Biofield Energy Treated Vitamin D3 on Bone Health Parameters in Human Bone Osteosarcoma Cells (MG-63)

Journal: Biochemistry and Molecular Biology PDF  

Published: 09-Feb-18 Volume: 3 Issue: 1

DOI:10.11648/j.bmb.20180301.12 ISSN: 2575-5064 (Print); 2575-5048 (Online)

Authors: Dezi Ann Koster, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Abstract

The study was aimed to evaluate the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters. The test items, were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Dezi Ann Koster and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Different parameters were performed for the evaluation of bone health like ALP, collagen, and bone mineralization in human bone osteosarcoma cells (MG-63). The cell viability (MTT) data showed the test samples were found as safe in the tested concentrations. The level of ALP was significantly increased by 431.44% and 436.87% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item, respectively at 1 µg/mL compared to the UT-DMEM + UT-Test item group. Further, the ALP level was significantly elevated by 163.34%, 44.91%, and 57.67% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL compared to the untreated. Collagen was significantly increased by 56.58%, 84.95%, and 43.27% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item, respectively at 0.1 µg/mL compared to the untreated. Further, the collagen level was significantly increased by 21.27%, 47.26%, and 63.1% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL compared to the untreated. Besides, the percent of bone mineralization was distinctly increased by 41.87% and 91.87% at 1 µg/mL in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item groups, respectively while, increased by 72.66% and 186.68% at 50 µg/mL in the UT-DMEM + BT-Test item and BT-DMEM + BT-Test item groups, respectively compared to the untreated. The percent of bone mineralization was distinctly increased by 101.21%, 57.76%, and 125.53% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item, respectively at 10 µg/mL compared to the untreated. Altogether, the Biofield Energy Treated vitamin D3 was significantly improved the bone health parameters and it could be an excellent alternative nutraceutical supplement against various bone-related disorders including osteoporosis, low bone density, osteogenesis imperfecta, Paget’s disease, rickets, osteomalacia, deformed bones, autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.

Improved Metabolism of Vitamin D3 in Human Osteoblasts Cells After Biofield Energy Healing Treatment

Journal: American Journal of Laboratory Medicine PDF  

Published: 12-Jan-18 Volume: 3 Issue: 1

DOI:10.11648/j.ajlm.20180301.13 ISSN: 2575-3878 (Print); 2575-386X (Online)

Authors: Vaibhav Rajan Parulkar, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Abstract

Bone formation, remodeling, mineralization, resorption, and healing processes involves a coordinated action of different cell types, which are very prevalent in the elderly population. The current study aimed to evaluate the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health. Vitamin D3 and DMEM medium as test items (TI), were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Vaibhav Rajan Parulkar and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were studied. The cell viability using MTT assay showed that the test items were found to be safe. ALP level was significantly increased by 304.6%, 237.2%, and 110.6% at 1, 10, and 50 µg/mL, respectively in BT-DMEM+UT-TI, while 356.9%, 186.2%, and 87.3% at 1, 10, and 50 µg/mL, respectively in the BT-DMEM+BT-TI group as compared to the untreated group. Collagen content was significantly increased up to 163.7%, 114.4%, and 22.7% at 0.1, 1, and 10 µg/mL, respectively in the UT-DMEM+BT-TI group, while 190.1%, 64.9%, and 50% increased collagen at 0.1, 1, and 10 µg/mL, respectively in the BT-DMEM+BT-TI group as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 330% and 121.5% at 0.1 and 1 µg/mL, respectively in the BT-DMEM+BT-TI group, while 146.7% and 305.9% at 0.1 and 1 µg/mL, respectively in the BT-DMEM+UT-TI group, as compared with the untreated group. In conclusion, the Biofield Energy Treated vitamin D3 and DMEM would play an important role to control the osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium, and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, Paget’s disease of bone, rickets, deformed bones, osteomalacia, bone and/or joint pain, increased frequency of fractures, osteoma, hormonal imbalance, stress, aging, bone loss and fractures.

Influence of Biofield Treated Vitamin D3 on Proliferation, Differentiation, and Maturation of Bone-Related Parameters in MG-63 Cell-Line

Journal: International Journal of Biomedical Engineering and Clinical Science PDF  

Published: 08-Feb-18 Volume: 4 Issue: 1

DOI:10.11648/j.ijbecs.20180401.12 ISSN: 2472-1298 (Print); 2472-1301 (Online)

Authors: Dimitrius Anagnos, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Abstract

The study was aimed to evaluate the effect of Consciousness Energy Healing-based vitamin D3 and DMEM medium on bone health parameters such as alkaline phosphatase (ALP), collagen, and bone mineralization in human bone osteosarcoma cells (MG-63). The test items (vitamin D3 and DMEM), were divided into two parts. One part of each sample was received the Biofield Energy Treatment by Dimitrius Anagnos and those samples were denoted as the Biofield Energy Treated (BT) samples, while the other parts of each sample were referred as the untreated test items (UT). The test samples were found as safe in the tested concentrations by MTT cell viability assay. The level of ALP was significantly increased by 74.86% in the BT-DMEM + BT-Test item and 40.17% in the UT-DMEM + BT-Test item group at 0.1 and 50 µg/mL, respectively compared to the UT-DMEM + UT-Test item group. Moreover, collagen level was significantly increased by 228.22%, 185.40%, and 256.69% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 0.1 µg/mL compared to the UT-DMEM + UT-Test item group. Further, the collagen level was significantly increased by 88.61% and 130.65% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item groups, respectively at 1 µg/mL compared to the untreated group. Apart from this, the percent of bone mineralization was distinctly enhanced by 98.61%, 111.67%, and 68.26% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 0.1 µg/mL compared to the untreated group. Additionally, the percentage of bone mineralization was significantly increased by 83.3%, 99.52%, and 50.26% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 1 µg/mL compared to the untreated group. Altogether, the Biofield Energy Treated vitamin D3 was significantly improved the bone health parameters and it could be an alternative approach for nutraceutical supplement to combat vitamin D3 deficiency and able to fight against various bone-related disorders including rickets, low bone density, osteomalacia, bone and joint pain, bone fractures, osteoporosis, osteoma, osteogenesis imperfecta, Paget’s disease, deformed bones, chondrodystrophia fetalis, stress management and prevention, autoimmune and inflammatory diseases, and anti-aging by improving overall health.