Life Sciences

This study tested the Null Hypothesis for the effect of BioField Energy applied to two separate crops under typical growing conditions, namely ginseng and organic blueberry in commercial plantings in Wisconsin and California, respectively. Following treatment to replicated plots in standard experimental design, data were collected at harvest for yield quantity and quality. Ginseng plants treated both pre-harvest and a combination of pre- and post-harvest showed market grade increases of 33.3% and 40.0%, respectively. Point of sale gross return for this crop is dependent upon tuber quality, and from these data the economics of these treatments were calculated. Based on stand adjusted yields and quality values, a combination of pre- and post-harvest treatment increased gross income by 57.4%. The second crop showed similar trends in positive responses. In the two blueberry varieties studied, Emerald treated plants showed 96% statistical increase in yield, while Jewel showed 31% increase. At the time of treatment, each variety was in a different stage of flowering. The Emerald variety was in the flowering stage, and Jewel was predominately in the fruiting stage. Both treated cultivars however demonstrated increased yield quantity and quality. The specific mechanisms that lead to these preliminary results need further investigation.

Purpose

While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiment on Staphylococcus epidermis [ATCC-13518], validate the effects of such energy transmitted through a person, Mr. Mahendra kumar Trivedi, which has produced an impact measurable in scientifically rigorous manner.

Methods

Staphylococcus epidermis strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain, the process taking about 3 minutes and were analyzed within 10 days after incubation. All tests were performed with the help of automation on the Microscan Walkaway System in Microbiology Laboratory – accredited by The College of American Pathologists.

Results

The results indicated that Mr.Trivedi’s energy has changed 7 of 27 biochemical characteristics of Staphylococcus epidermis along with significant changes in susceptibility pattern in 8 of 29 antibiotics. The Biotype number has changed from the original control strain giving rise to 2 different biotypes in treated samples while the external energy /treatment given was the same for all treated samples suggestive of random polymorphism as analyzed through the automated machine.

Conclusions

These results cannot be explained by current theories of science, and indicate a potency in Mr. Trivedi’s energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner.

In lyophilized state, biochemical and enzymatic characteristics could be altered.

Background :
While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Yersinia enterocolitica [ATCC –23715], report the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner.
Methods:
Yersinia enterocolitica strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain and were analyzed within 10 days after incubation.
Results:
The results indicated that Mr.Trivedi's energy has changed 20 of 33 biochemical characteristics of Yersinia enterocolitica along with significant changes in susceptibility pattern in 15 of 32 antibiotics. The Biotype number has changed from the original control strain giving rise to 2 different biotypes in treated samples while the external energy /treatment given was the same for all treated samples suggestive of random polymorphism as analyzed through an automated machine.
Conclusions:
These results cannot be explained by current theories of science, and indicate a potency in Mr.Trivedi's energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner.
In lyophilized state, biochemical and enzymatic characteristics could be altered.

Background :
While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Enterococcus faecalis [ATCC –51299], report the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner.
Methods:
Enterococcus faecalis strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain, the process taking about 3 minutes and were analyzed within 10 days after incubation. All tests were performed with the help of automation on the Microscan Walkaway System in Microbiology Laboratory ­ accredited by The College of American Pathologists
Results:The results indicated that Mr.Trivedi’s energy has changed 9 of 27 biochemical characteristics of Enterococcus faecalis along with significant changes in susceptibility pattern in 5 of 31 antibiotics. The Biotype number has changed from the original control strain giving rise to 2 different biotypes in treated samples while the external energy /treatment given was the same for all treated samples suggestive of random polymorphism as analyzed through the automated machine.
Conclusions:
These results cannot be explained by current theories of science, and indicate a potency in Mr.Trivedi’s energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner.
In lyophilized state, biochemical and enzymatic characteristics could be altered.

Global emergence of Pseudomonas fluorescens (P. fluorescens) displays a mechanism of resistance to all existing antimicrobials. Due to its strong ability to acquire resistance, there is a need of some alternative treatment strategy. Objective of this study was to investigate the effect of biofield treatment on antimicrobial sensitivity pattern of P. fluorescens. P. fluorescens cells were procured from MicroBioLogics in sealed packs bearing the American Type Culture Collection (ATCC 49838) number. Two sets of ATCC samples were taken in this experiment and denoted as A and B. ATCC-A sample was revived and divided into two groups (Gr) i.e. Gr.I (control) and Gr.II (revived); likewise, ATCC-B was labeled as Gr.III (lyophilized). Gr.II and III were given biofield treatment and were measured by MicroScan Walk-Away® system before and after treatment. Parameters studied in experiment were antimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical reactions, and biotype number of both control and treatment groups using MicroScan Walk-Away® system. Experimental results showed antimicrobials such as cefepime, cefotaxime, ceftazidime, ceftriaxone, ciprofloxacin, piperacillin, tetracycline, and tobramycin showed altered sensitivity and MIC values in treated group as compared to control. Biochemical reactions showed positive reaction in malonate, melibiose, nitrate, galactosidase, ornithine, raffinose, sorbitol, sucrose, tobramycin and Voges-Proskauer in Gr.II. Arabinose, colistin, glucose, and rhaminose also showed positive reactions in Gr.II on day 10 while arginine and cetrimide showed negative reaction in Gr.III as compared to control. Biochemical tests results revealed a change in biotype number in Gr.II (34101173, day 5), (77103177, a very rare biotype on day 10) and Gr.III (40000043) as compared to control (02041722). Organism was identified as Enterobacter cloacae (GrII, day 10) and Vibrio fluvialis (Gr.III, day 10) with respect to control. These findings suggest that biofield treatment made significant alteration in sensitivity pattern, MIC values, and biotype number of P. fluorescens.

Burkholderia cepacia (B. cepacia) is an opportunistic, Gram negative pathogen which causes infection mainly in immunocompromised population and associated with high rate of morbidity and mortality in cystic fibrosis patients. Aim of the present study was to analyze the impact of biofield treatment on multidrug resistant B. cepacia. Clinical sample of B. cepacia was divided into two groups i.e. control and biofield treated. The analysis was done after 10 days of treatment and compared with control group. Control and treated group were analyzed for susceptibility pattern, MIC value, biochemical studies and biotype number using MicroScan Walk-Away® system. Sensitivity assay results showed a change in pattern from resistant to intermediate in aztreonam, intermediate to resistant in ceftazidime, ciprofloxacin, imipenem, and levofloxacin while sensitive to resistant in meropenem and piperacillin/ tazobactam. The biofield treatment showed an alteration in MIC values of aztreonam, ceftazidime, chloramphenicol, ciprofloxacin, imipenem, levofloxacin, meropenem, piperacillin/tazobactam and tetracycline. Biochemical reactions of treated group showed negative reaction in colistin, lysine, and ornithine while positive reactions to acetamide, arginine, and malonate as compared to control. Overall results showed an alteration of 38.9% in susceptibility pattern, 30% in MIC values of tested antimicrobials and 18.2% change in biochemical reaction after biofield treatment. A significant change in biotype number (02063736) was reported with green pigment as special characteristics after biofield treatment as compared to control (05041776) group with yellow pigment. In treated group, a new species was identified as Pseudomonas aeruginosa, as compared to control. Study findings suggest that biofield treatment has a significant effect on the phenotypic character and biotype number of multidrug resistant strain of B. cepacia.

This study tested the Null Hypothesis for the effect of biofield treatment when used for control of nematode on carrot crops, Daucus carota, under typical growing conditions in year 2012 at Guadalupe, California, USA. Following biofield treatment, carrot seeds were planted in replicate plots with mechanical seeder and their development was recorded compared to control seed growth from untreated plots and plots treated with the commercial standard nematicide, further Vydate L was applied three times at rates of 1 and 0.5 gal/A, at 1, 18 and 35 days after seeding, respectively. At 70 and 109 days after the first application root galling severity in biofield treated crops was reduced by 54% and 22% respectively as compared to untreated while the Vydate response showed 0% and 25% control, respectively. Plots planted with biofield treated seeds resulted in the greatest number and weight of marketable carrot roots. Total yield and gross return were greatest in biofield treated group producing an approximately 33% increase over the untreated controls and 15% increase over those treated with the commercial standard, Vydate L. Vitamin A (beta carotene) was significantly greater (6512 IU/100 g) in biofield treated carrots compared with both the untreated controls (4941) and the commercial standard (5143). The results concluded that, Biofield treatment caused the numerical improvement in yield along with nematode control in carrots however, caused statistically significant increase in Vitamin A content.

Fungi are the group of eukaryotic organisms such as yeast, mold, and mushrooms. The present work investigated the impact of biofield treatment on different pathogenic species of fungi in relation to antifungal sensitivity pattern. Each fungal sample was divided into three parts: C, control; T1, treatment (revived); T2 treatment (lyophilized). Treatment groups received the biofield treatment, and control group was remained as untreated. Mini-API ID32C strip employed for evaluation of antifungal sensitivity and minimum inhibitory concentration (MIC). The results showed that sensitivity of Candida albicans in T1 cells was changed against itraconazole from intermediate (I) to resistance (R) on day 10. The Candida kefyr exhibited a change in susceptibility against itraconazole in T2 cell from S?I, on day 10. Likewise, Candida krusei showed the alterations in sensitivity against two antifungal drugs: fluconazole from S?I (T1 on day 10) and itraconazole S?I (T1 and T2 on all assessment days). The Cryptococcus neoformans changed from S?I in T1 cell on day 5 and 10, against itraconazole. Sensitivity of Candida tropicalis was also altered from I?R against flucytosine (T1 and T2, on all assessment days). Similarly, Saccharomyces cerevisae altered from S?I (T1) and S?R (T2) on day 10. The MIC values of antifungal drugs were altered in the range of 2-8 folds, as compared to the control. Fungal identification data showed the significant changes in species similarity of few tested fungi as C. albicans changed from 91.9% to 98.5 and 99.9% in T1 and T2 cells, respectively on day 10. C. krusei was changed from 97.9% to 85.9% (T2 day 10), and C. tropicalis was altered from 88.7% to 99.6% (T1 day 5) and 99.0% (T2). These findings suggest that biofield treatment could be applied to alter the susceptibility pattern of antifungal drug therapy in future.

Global emergence of Acinetobacter baumannii (A. baumannii) displays a mechanism of resistance to all existing antimicrobials. Objective of this study was to investigate the effect of biofield treatment on antimicrobial sensitivity pattern, minimum inhibitory concentration (MIC), biochemical reactions and biotype number of A. baumannii. A. baumannii cells were procured from MicroBioLogics in sealed packs bearing the American Type Culture Collection (ATCC 19606) number and stored according to the recommended storage protocols until needed for experiments. Two sets of ATCC samples were taken in this experiment and denoted as A and B. ATCC-A sample was revived and divided into two parts i.e. Gr.I (control) and Gr.II (revived) analyzed on day 5 and 10, respectively; likewise, ATCC-B was labeled as Gr.III (lyophilized) and was assessed on day 10. Gr.II and III were treated with Mr. Trivedi’s biofield and were analyzed for its antimicrobial sensitivity, MIC value, biochemical reactions and biotype number with respect to control. Experimental results showed the impact of biofield treatment directly onto the revived and lyophilized form of A. baumannii and found alteration both in qualitative and quantitative aspect as compared with untreated groups. These results showed altered sensitivity pattern of antimicrobials in biofield treated group as compared to control. Apart from altered MIC values, changes were also observed in biotype number of revived treated group as compared to control. These findings suggest that biofield treatment can prevent the emergence of absolute resistance of existing antimicrobials to A. baumannii.

Proteus mirabilis (P. mirabilis) is widespread in nature, mainly found in soil, water, and the flora of human gastrointestinal tract. The current study was attempted to investigate the effects of Mr. Trivedi’s biofield energy treatment on P. mirabilis both in lyophilized as well as revived state for antimicrobial susceptibility, biochemical characteristics, and biotype. P. mirabilis cells were procured from MicroBioLogics Inc., USA, in a sealed pack bearing the American Type Culture Collection (ATCC 25933) number and stored according to the recommended storage protocol until needed for experiments. Two sets of ATCC samples were taken in this experiment and denoted as A and B. The ATCC A sample was revived and divided into two parts Gr.I (control) and Gr.II (revived); likewise, the ATCC B was labeled as Gr.III (lyophilized). Group II and III were given with biofield treatment. All experimental parameters were studied using automated MicroScan Walk-Away® system. The result of antimicrobial susceptibility and minimum inhibitory concentration showed 6.67% and 9.38% alteration, respectively in treated cells of P. mirabilis as compared to the control. In addition, the overall biochemical reactions were significantly altered (42.42%) in the treated groups with respect to the control. Moreover, biotype number was changed in the treated cells, Gr. II, day 5 (40061546) and day 10 (77365764), while without alteration of organism as compared to the control (40061544; Proteus mirabilis). The results suggested that biofield treatment has an impact on P. mirabilis in revived state predominately.

Food production needs to increase to satisfy the demand due to increasing human population worldwide. To minimize this food crisis, an increase in the rice production is necessary in many countries. The current study was undertaken to evaluate the impact of Mr. Trivedi’s biofield energy treatment on rice (Oryza sativa) for its growth-germination of seedling, glutathione (GSH) content in seedling and mature plants, indole acetic acid (IAA) content in shoots and roots and DNA polymorphism by random amplified polymorphic-DNA (RAPD). The sample of O. sativa cv, 644 was divided into two groups. One group was remained as untreated and coded as control, while the other group was subjected to Mr. Trivedi for biofield energy treatment and denoted as treated sample. The growth-germination of O. sativa seedling data exhibited that the biofield treated seeds was germinated faster on day 3 as compared to control (on day 5). The shoot and root length of seedling was slightly increased in the treated seeds of 10 days old with respect to untreated seeds. Moreover, the plant antioxidant i.e. GSH content in seedling and in mature plants was significantly increased by 639.26% and 56.24%, respectively as compared to untreated sample. Additionally, the plant growth regulatory constituent i.e. IAA level in root and shoot was significantly (p<0.05) increased by 106.90% and 20.35%, respectively with respect to control. Besides, the DNA fingerprinting data using RAPD, revealed that the treated sample showed an average range of 5 to 46% of DNA polymorphism as compared to control. The overall results envisaged that the biofield energy treatment on rice seeds showed a significant improvement in germination, growth of roots and shoots, GSH and IAA content in the treated sample. In conclusion, the treatment of biofield energy on rice seeds could be used as an alternative way to increase the production of rice.

Introduction: Complementary and alternative medicine such as biofield energy therapies are highly popular in biomedical health care. The study evaluates the impact of Mr. Trivedi’s biofield energy treatment on Pseudomonas aeruginosa (P. aeruginosa) to evaluate its phenotypic and genotypic characteristics.

Methods: P. aeruginosa ATCC 10145 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to biofield treatment and analyzed for antibiogram, biochemical reactions, and biotype number using automated MicroScan Walk-Away® system on day 10. The treated sample was evaluated for DNA polymorphism by Random Amplified Polymorphic DNA (RAPD) and 16S rDNA sequencing to establish the phylogenetic relationship, the epidemiological relatedness and genetic characteristics.

Results: Data showed altered sensitivity pattern in antibiotic cefotaxime from intermediate to decreased ?-lactamases activity, with four-fold decreased minimum inhibitory concentration (MIC), i.e. 32 to ?8 µg/mL as compared to control. Similarly, cefotetan and extended-spectrum-?-lactamases (ESBL-b Scrn) showed decrease in MIC values as compared to the control group. Nitrate reported for negative biochemical reaction i.e. positive (+) to negative (-) after biofield treatment on P. aeruginosa. The biotyping showed a change in biotype number (02063722) as compared to the control (02063726), without altering the microorganism. RAPD analysis showed an average range of 30 to 50% of polymorphism, while 16S rDNA sequencing analyzed treated sample as Pseudomonas aeruginosa (GenBank Accession Number: EU090892) with 99% identity of gene sequencing data.

Conclusion: These results suggest that Mr. Trivedi’s unique biofield energy treatment on P. aeruginosa has an impact to alter the antimicrobial sensitivity pattern and MIC values, thus it can be used as an alternate integrative approach of energy medicine in near future.

The present study was aimed to evaluate the effect of Consciousness Energy Healing Treatment on plant callus of Mandukparni (Centellaasiatica), Katsarika (Barleriacristata), and Amla (Phyllanthus emblica) in Murashige & Skoog liquid medium (MS) for its growth and yield on day 7. The plant callus of all the three plants were divided into two parts, one part received Biofield Energy Treatment remotely (known as The Trivedi Effect®-Energy of Consciousness Healing Treatment) by a renowned Biofield Energy Healer, Gopal Nayak and denoted as Biofield Energy Treated group, while another part did not receive any treatment and defined as control. Plants callus were observed on day 7 after treatment and visualized under phase contrast microscope for cell growth, captured photomicrographs along with wet weight compared with day 0. Results suggested that three plants viz. Mandukparni, Katsarika, and Amla callus after 7 days showed a significant improved growth rate as compared with the respective untreated groups. The weight of the callus growth (in mg) after Biofield Energy Treatment among Mandukparni, Katsarika, and Amla callus on day 7 was 630, 782, and 920 mg, respectively. However, the percentage increase in weight of the callus in Mandukparni, Katsarika and Amla was 11.1%, 52.4%, and 68.5%, respectively than untreated. Hence, results concluded a significant growth in callus weight after Biofield Energy Treatment, which suggested that it could be used as complementary and alternate approach in order to produce most high-value phytoconstituents from plant callus that can be used for making various drugs, food flavoring and colouring agents.

The objective of the present study was to evaluate the effect of Biofield Energy Healing Treatment on the plant callus of Mandukparni (Centella asiatica), Katsarika (Barleria cristata), and Amla (Phyllanthus emblica) in Murashige & Skoog liquid medium (MS) for their yield on day 7. All the three plant callus and MS liquid media were divided into two parts, one part was received Biofield Energy Treatment (known as The Trivedi Effect®-Energy Of Consciousness Healing Treatment) by a renowned Biofield Energy Healer, Alice Branton through the Healer’s unique Energy Transmission process remotely and defined as Biofield Energy Treated group. Another part did not receive any treatment and defined as the control group. On day 7 after the treatment, the flasks were observed and visualized under the phase contrast microscope for monitoring the cell growth and photomicrographs were captured at 200X magnification along with the wet weight of the callus cultures. Experimental data suggested that all the three plants viz. Mandukparni, Katsarika, and Amla callus showed significantly improved growth rate after seven days as compared with the respective untreated groups. The growth of the callus (weight in mg) of Mandukparni, Katsarika and Amla callus after 7 days of the Biofield Energy Treatment was 289.7, 348.5, and 512 mg respectively. However, the percentage increase in weight of the callus in Mandukparni, Katsarika, and Amla was 3.3%, 7.7%, and 79.6%, respectively as compared with the untreated group. Hence, the results indicated that there was a significant growth observed after The Trivedi Effect®-Energy of Consciousness Treatment in the tested plant stem cells. It can be concluded that The Trivedi Effect® would be used as a complementary and alternative treatment to maximize most valuable natural products yield and quality best quality such as herbal medicinal plants, food and dietary supplements, flavoring and coloring agents, cosmetics and fragrance products, and many more.

Pogostemoncablin is a known aromatic plant which is cultivated for its essential oil widely applicated in perfumery and cosmetic industries. In the present study, the effect of biofield treatment was studied on the growth of P. cablin. For this study an in-vitro culture system was set up in two groups, viz., control and treatment, each of which was derived from three different explant sources, namely leaf, node and petiole. Further these in-vitro plantlets were hardened and transferred to external environment. The stomatal cells and epidermal hair growth were also studied at various morphogenetic stages. The study revealed that a single spell of biofield energy treatment produced significant increase in growth in treated group throughout all the morphogenetic phases from in-vitro to in vivo level. A remarkable increase in stomatal cells and epidermal hair was also seen in treated group.

Quantum mechanics was developed when human energies of consciousness were found to influence observations at the scale of elementary particles, here referred as non-contact biofield treatment or biofield energies . Quantum mechanics has also proved efficacious in biological processes. The present experiments found an enhanced and significant impact of the biofield treatment on adaptive micropropagation response and callus induction of two plant species, Withania somnifera and Amaranthus dubius. The enhancement was perhaps due to greater focus on adaptation rather than specific mechanisms, showing high potential including at biochemical and genetic levels. Possible reasons for the enhancement are discussed and a possible model is presented, consistent with current scientific theory.