Antibiogram, Biochemical Reactions and Genotyping Characterization of Biofield Treated Staphylococcus aureus

Journal: American Journal of Bioscience PDF  

Published: 15-Oct-15 Volume: 3 Issue: 6

DOI:10.11648/j.ajbio.20150306.13 ISSN: 2330-0159 (print) 2330-0167 (online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Abstract

Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen

Journal: Journal of Clinical & Medical Genomics PDF  

Published: 30-Sep-15 Volume: 3 Issue: 1

DOI:10.4172/2472-128X.1000129 ISSN: 2472-128X

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal and Snehasis Jana

Abstract

Investigation of Biofield Treatment on Antimicrobial Susceptibility, Biochemical Reaction Pattern and Biotyping of Enteropathogenic Multidrug-Resistant Escherichia coli Isolates

Journal: General Medicine: Open Access PDF  

Published: 31-Aug-15 Volume: S2 Issue: 2

DOI:10.4172/2327-5146.1000S2-002 ISSN: 2327-5146

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Harish Shettigar , Mayank Gangwar and Snehasis Jana

Abstract

Study background: Multidrug resistant Escherichia coli (MDR E. coli) has become a major health concern, and failure of treatment leads to huge health burden. Aim of the present study was to determine the impact of Mr. Trivedi’s biofield treatment on E. coli.
Methods: Four MDR clinical lab isolates (LSs) of E. coli (LS 8, LS 9, LS 10, and LS 11) were taken and divided into two groups i.e. control and biofield treated. Control and treated samples were identified with respect to its antimicrobial sensitivity assay, biochemical study and biotype number using MicroScan Walk-Away® system. The analysis was done on day 10 after biofield treatment and compared with its respective control group.

Results: Antimicrobial sensitivity assay showed 50% alteration in sensitivity of total tested antimicrobials in treated group of MDR E. coli isolates. MIC results showed the alteration in MIC of about 40.63% antimicrobials out of thirty two tested antimicrobials, after biofield treatment in clinical isolates of E. coli. Ticarcillin/k-clavulanate showed improved sensitivity (R → I) with decreased MIC value in LS 9 as compared to control. A fourfold and twofold decreased in MIC values were reported in case of piperacillin/tazobactam (in LS 9) and chloramphenicol (in LS 8), respectively as compared to respective control. Biochemical study showed a 39.39% alteration in biochemical reactions after treatment among four isolates of E. coli as compared to control. A significant change in biotype numbers were reported in three clinical isolates (i.e. LS 8, LS 9, and LS 11) of MDR E. coli as compared to control. On the basis of changed biotype number (7774 5272) after biofield treatment, organism with maximum probability was identified as Enterobacter aerogenes in LS 8 as compared to control, (E. coli, 7711 5012).

Conclusion: Overall results suggest that Mr Trivedi’s biofield treatment has a significant effect on altering the antimicrobial sensitivity, biochemical reactions and biotype number of MDR isolates of E. coli.

Antimicrobial Sensitivity, Biochemical Characteristics and Biotyping of Staphylococcus saprophyticus: An Impact of Biofield Energy Treatment

Journal: Women’s Health Care PDF  

Published: 01-Oct-15 Volume: 4 Issue: 6

DOI:10.4172/2167-0420.1000271 ISSN: 2167-0420

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak , Sambhu Charan Mondal and Snehasis Jana *

Abstract

Effect of Biofield Treated Energized Water on the Growth and Health Status in Chicken (Gallus gallus domesticus)

Journal: Poultry, Fisheries & Wildlife Sciences PDF  

Published: 30-Sep-15 Volume: 3 Issue: 2

DOI:10.4172/2375-446X.1000140 ISSN: 2375-446X

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak , Sambhu Charan Mondal and Snehasis Jana *

Abstract

Antibiogram of Multidrug-Resistant Isolates of Pseudomonas aeruginosa after Biofield Treatment

Journal: Infectious Diseases & Therapy PDF  

Published: 25-Oct-15 Volume: 3 Issue: 5

DOI:10.4172/2332-0877.1000244 ISSN: 2332-0877

Authors: Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, Gangwar M and Jana S*

Abstract

In recent years, prevalence of multidrug resistance (MDR) in Pseudomonas aeruginosa (P. aeruginosa) has been noticed with high morbidity and mortality. Aim of the present study was to determine the impact of Mr. Trivedi’s biofield treatment on MDR clinical lab isolates (LS) of P. aeruginosa. Five MDR clinical lab isolates (LS 22, LS 23, LS 38, LS 47, and LS 58) of P. aeruginosa were taken and divided into two groups i.e. control and biofield treated. Control and treated group were analyzed for antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), biochemical study and biotype number using MicroScan Walk-Away® system. The analysis was done on day 10 after biofield treatment as compared with control group. Antimicrobial sensitivity assay showed 60% alteration in sensitivity of tested antimicrobials in MDR isolates of P. aeruginosa after biofield treatment. MIC results showed an alteration in 42.85% tested antimicrobials out of twenty eight after biofield treatment in five isolates of MDR P. aeruginosa. Biochemical study showed a 48.48% change in tested biochemical reactions out of thirty three as compared to control. A significant change in biotype numbers was reported in three clinical lab isolates of MDR P. aeruginosa out of five, after biofield treatment as compared to respective control. On the basis of changed biotype number (7302 0052) in biofield treated LS 23, new organism was identified as Citrobacter freundii as compared to control (0206 3336). A very rare biotype number (7400 4263) was found in biofield treated LS 38, as compared to control (0206 3736). Study results suggest that biofield treatment on lab isolates of MDR P. aeruginosa has significant effect on the antimicrobial sensitivity, MIC values, biochemical reactions and biotype number. Biofield treatment might prevent the emergence of absolute resistance pattern of useful antimicrobials against MDR isolates of P. aeruginosa.

Physical, Thermal, and Spectroscopic Characterization of Biofield Energy Treated Potato Micropropagation Medium

Journal: American Journal of Bioscience and Bioengineering PDF  

Published: 09-Nov-15 Volume: 3 Issue: 5

DOI:10.11648/j.bio.20150305.24 ISSN: 2328-5885 (Print) 2328-5893 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Khemraj Bairwa, Snehasis Jana

Abstract

Potato Micropropagation Medium (PMM) is the growth medium used for in vitro micropropagation of potato tubers. The present study was intended to assess the effect of biofield energy treatment on the physical, thermal and spectroscopic properties of PMM. The study was attained in two groups i.e. control and treated. The control group was remained as untreated, while the treated group was received Mr. Trivedi’s biofield energy treatment. Finally, both the samples (control and treated) were evaluated using various analytical techniques such as X-ray diffractometry (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis- differential thermal analysis (TGA-DTA), UV-Vis spectrometry, and Fourier transform infrared (FT-IR) spectroscopy. The XRD analysis showed the crystalline nature of both control and treated samples of PMM. The X-ray diffractogram showed the significant increase in the intensity of XRD peaks in treated sample as compared to the control. The XRD analysis revealed 6.64% increase in the average crystallite size of treated PMM with respect to the control. The DSC analysis showed about 8.66% decrease in the latent heat of fusion in treated sample with respect to the control. The TGA-DTA analysis exhibited about 4.71% increase in onset temperature of thermal degradation after biofield treatment with respect to the control, while the maximum thermal degradation temperature (Tmax) was also increased (5.06%) in treated sample with respect to the control. This increase in Tmax might be correlated with increased thermal stability of treated sample as compared to the control. The UV spectroscopic study showed the slight blue shift in λmax of treated sample with respect to the control. FT-IR spectrum of control PMM showed the peak at 3132 cm-1 (C-H stretching) that was observed at higher wavenumber i.e. at 3161 cm-1 in the treated sample. Other vibrational peaks in the treated sample were observed in the similar region as that of the control. Altogether, the XRD, DSC, TGA-DTA, UV-Vis, and FT-IR analysis suggest that Mr. Trivedi’s biofield energy treatment has the impact on physicochemical properties of PMM. This treated PMM might be more effective as a micropropagation medium as compared to the control.

Use of Energy Healing Medicine Against Escherichia coli for Antimicrobial Susceptibility, Biochemical Reaction and Biotyping

Journal: American Journal of Bioscience and Bioengineering PDF  

Published: 09-Nov-15 Volume: 3 Issue: 5

DOI:10.11648/j.bio.20150305.23 ISSN: 2328-5885 (Print) 2328-5893 (online)

Authors: Mahendra Kumar Trivedi, Alice Branton , Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Abstract

In vitro Evaluation of Biofield Treatment on Viral Load Against Human Immunodeficiency-1 and Cytomegalo Viruses

Journal: American Journal of Health Research PDF  

Published: 09-Nov-15 Volume: 3 Issue: 6

DOI:10.11648/j.ajhr.20150306.14 ISSN: 2330-8788 (Print) 2330-8796 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Abstract

Viral load quantification is the amount of particular viral DNA or RNA in a blood samples. It is one of the surrogate biomarker of AIDS. High viral load indicates that the immune system is failed to fight against viruses. The aim of this study was to evaluate the impact of biofield treatment on HIV-1 and HCMV in terms of viral loads as surrogate marker. The viral load assay was performed on stored stock cultures of HIV infected human plasma samples before and after 7 days of biofield treatment using Roche COBAS® AMPLICOR analyzer. Viral load (HIV-1 RNA and HCMV DNAaemia) was considered as surrogate marker for assessment of the impact of Mr. Trivedi’s biofield treatment in HIV infected stored plasma samples. The viral load quantification of HIV-1 RNA in infected stored plasma samples was significantly reduced by 65% in biofield treated group as compared to control. Additionally, viral load of HCMV DNAaemia in infected stored plasma samples was also reduced by 80% in the biofield treated group as compared to control. Because, children are more prone to HCMV infection and adults are generally liable to suffer from HIV-1 infection. As the biofield treatment has reduced HCMV DNAaemia, it could be beneficial for HIV infected children populations. Altogether, data suggest that biofield treatment has significantly reduced the viral load quantification in HIV-1 and HCMV infected stored plasma samples and could be a suitable alternative treatment strategy for AIDS patients in near future.

Antibiogram, Biochemical Reactions and Biotyping of Biofield Treated Providencia rettgeri

Journal: American Journal of Health Research PDF  

Published: 09-Nov-15 Volume: 3 Issue: 6

DOI:10.11648/j.ajhr.20150306.15 ISSN: 2330-8788 (Print) 2330-8796 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Abstract

Providencia rettgeri (P. rettgeri) is the key organism for gastrointestinal tract infections due to its high virulence properties. The current study was designed to investigate the effect of Mr. Trivedi’s biofield energy treatment on P. rettgeri in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. The lyophilized strain of P. rettgeri (ATCC 9250) was divided into two parts, Group (Gr.) I: control and Gr. II: treatment. After biofield treatment, Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 162 after revival (Study I). The revived sample of Gr. IIB was retreated on day 162 (Study II), and divided into three separate tubes. Tube 1 was analyzed on day 5, likewise, tube 2 and 3 were analyzed on day 10 and 15, respectively after their sub-culturing. All the experimental parameters were studied using automated MicroScan Walk-Away® system. The antimicrobial susceptibility and minimum inhibitory concentration were significantly improved by 71.43%, out of twenty-eight and 56.25%, out of thirty-two, respectively in the treated cells of P. rettgeri as compared to the control. The biochemical reactions also showed the significant (60.61%) alteration in the treated sample with respect to control. The biotype numbers were substantially changed in all the treated groups as compared to the control. Moreover, the organism was changed as Proteus mirabilis in all the treated groups except in Gr. IIA, as compared to the control. These results suggested that biofield treatment has a significant impact on P. rettgeri in lyophilized as well as revived state.

Improved Susceptibility Pattern of Antimicrobials Using Vital Energy Treatment on Shigella sonnei

Journal: American Journal of Internal Medicine PDF  

Published: 09-Nov-15 Volume: 3 Issue: 6

DOI:10.11648/j.ajim.20150306.13 ISSN: 2330-4316 (Print) 2330-4324 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Abstract

Complementary and alternative medicine (CAM) has become increasingly popular and reported for countless benefits in biomedical health care systems. The study assessed the potential impact of The Trivedi Effect® (biofield energy) on Shigella sonnei for changes in antimicrobial sensitivity, biochemical study, and biotype number using MicroScan Walk-Away® system. The cells were obtained from MicroBioLogics Inc., USA bearing the American Type Culture Collection (ATCC 9290) number, and divided into two groups, Group (Gr.) I: control and Gr. II: treated. Gr. II was subjected to Mr. Trivedi’s biofield energy treatment and further subdivided into two sub-groups, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 160 (Study I). The Gr. IIB sample was retreated on day 160 (Study II), and was divided into three separate tubes as first, second and third tube, which were analyzed on day 5, 10 and 15, respectively. Results showed that 35% (7 out of 20) antimicrobials were reported with improved sensitivity profile. Moreover, the minimum inhibitory concentration study showed that 56.25% (18 out of 32) tested antimicrobials were reported with decreased concentration by two to four-fold as compared with the control after biofield treatment. The effect was further analyzed and sustained in the biochemical study, where 57.57% (19 out of 33) tested biochemicals showed altered reaction pattern as compared with the control. The biotype study showed an alteration in the biotype number in all the experimental treated groups as compared to the control. Encouraging results suggests that bioenergy healing treatment as an integrative medicine against S. sonnei would be a better and safe treatment approach in near future.

Physicochemical Characterization of Biofield Treated Orchid Maintenance/Replate Medium

Journal: Journal of Plant Sciences PDF  

Published: 16-Nov-15 Volume: 3 Issue: 6

DOI:10.11648/j.jps.20150306.11 ISSN: 2331-0723 (Print) 2331-0731 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ragini Singh, Snehasis Jana

Abstract

Orchids are used worldwide for indoor decoration, vanilla production, and beverage preparation. They are also reported for their therapeutic efficacy in brain-related problems. The in vitro micropropagation technique was used for their propagation using the orchid maintenance/replate (OMR) medium. The current study was based on analysing the effect of biofield energy treatment on the physicochemical properties of OMR medium. A part of the sample was treated with Mr. Trivedi’s biofield energy; various physicochemical properties were analyzed and compared with the untreated (control) part. The X-ray diffraction analysis revealed the decrease in crystallite size of treated sample (132.80 nm) as compared to the control (147.55 nm). The particle size analysis revealed 20.78% increase in average particle size and 39.29% increase in d99 (size below which 99% particles are present) of the treated OMR medium as compared to the control. Moreover, the surface area of the treated sample was reduced by 3.9%, supporting the data of particle size analysis. The thermal analysis studies revealed an increase in the thermal stability of the treated OMR medium as compared to the control. The analysis was done by using differential scanning calorimetry that showed increase in melting point (1.23%) and latent heat of fusion (135.7%); and thermogravimetric analysis that reported increase in onset temperature and maximum thermal degradation temperature of the treated sample as compared to the control. Besides, the CHNSO analysis revealed the increase in percentage of nitrogen (22.22%) as well as the presence of sulphur in the treated sample. The Fourier transform infrared and UV-visible spectroscopy also showed the differences in the spectra of the treated sample as compared to the control OMR medium. Hence, the overall data revealed the impact of biofield energy treatment on the physicochemical properties of the treated sample that might be used in better way in the in vitro culture techniques as compared to the control sample.

Physicochemical and Spectroscopic Characterization of Biofield Energy Treated Gerbera Multiplication Medium

Journal: Plant PDF  

Published: 09-Nov-15 Volume: 3 Issue: 6

DOI:10.11648/j.plant.20150306.11 ISSN: 2331-0669 (Print) 2331-0677 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton , Dahryn Trivedi, Gopal Nayak, Ragini Singh, Snehasis Jana

Abstract

The micropropagation technique is used for Gerbera species due to their high demand all over the world as the decorative potted plants and cut flowers. The present study was done to investigate the impact of biofield energy treatment on the physicochemical properties of gerbera multiplication medium. A part of the sample was treated with Mr. Trivedi’s biofield energy, and the other part was kept as untreated and termed as the control sample. Both the parts were subsequently analysed for their physical, thermal and spectral properties using X-ray diffraction (XRD), particle size analysis, surface area analysis, thermogravimetric analysis (TGA), elemental analysis, and Fourier transform infrared (FT-IR) spectroscopy. The XRD results showed 13.98% increase in crystallite size of treated sample (104.01 nm) as compared to the control (91.25 nm). The particle size data revealed an increase in d50 (average particle size) and d99 (size below which 99% particles are present) by 72.57% and 42.26%, respectively of the treated sample as compared to the control. Moreover, the surface area of the treated sample was reduced from 0.694 m2/g (control) to 0.560 m2/g in the treated sample. The TGA data showed the increase in onset temperature along with the reduction in the percent weight loss of the treated sample as compared to the control. Besides, the elemental analysis revealed the significant decrease in the percentage of nitrogen (10.47%) and hydrogen (9.35%) as well as the presence of sulphur in the treated sample. The FT-IR results showed the differences in the IR frequencies corresponding to pyridine ring and N-H2 deformation of the treated sample as compared to the control. Hence, the overall data revealed that the biofield energy treatment had a significant impact on the physicochemical properties of the treated sample that might help to improve its uses in the in vitro tissue culture techniques as compared to the control sample.

Evaluation of Plant Growth Regulator, Immunity and DNA Fingerprinting of Biofield Energy Treated Mustard Seeds (Brassica juncea)

Journal: Agriculture, Forestry and Fisheries PDF  

Published: 09-Nov-15 Volume: 4 Issue: 6

DOI:10.11648/j.aff.20150406.16 ISSN: 2328-563X (Print) 2328-5648 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Abstract

Among the oilseeds grown around the world, mustard is one of the important crop worldwide due to its wide adaptability and high yielding capacity. Owing to the importance of its utilities as condiment, cooking oil and some medical aids, the demand for its seed production is too high. The present study was carried out to evaluate the impact of Mr. Trivedi’s biofield energy treatment on mustard (Brassica juncea) for its growth-germination of seedling, glutathione (GSH) content in leaves, indole acetic acid (IAA) content in shoots and roots and DNA polymorphism by random amplified polymorphic-DNA (RAPD). The sample of B. juncea was divided into two groups. One group was remained as untreated and coded as control, while the other group was subjected to Mr. Trivedi’s biofield energy treatment and referred as the treated sample. The growth-germination of B. juncea seedling data exhibited that the biofield treated seeds were germinated faster on day 5 as compared to the control (on day between 7-10). The shoot and root length of seedling were slightly increased in the treated seeds of 10 days old with respect to untreated seedling. Moreover, the major plant antioxidant i.e. GSH content in mustard leaves was significantly increased by 206.72% (p<0.001) as compared to the untreated sample. Additionally, the plant growth regulatory constituent i.e. IAA level in root and shoot was increased by 15.81% and 12.99%, respectively with respect to the control. Besides, the DNA fingerprinting data using RAPD revealed that the treated sample showed an average 26% of DNA polymorphism as compared to the control. The overall results envisaged that the biofield energy treatment on mustard seeds showed a significant improvement in germination, growth of roots and shoots, GSH and IAA content in the treated sample. In conclusion, the biofield energy treatment of mustard seeds could be used as an alternative way to increase the production of mustard.

Antibiogram of Biofield-Treated Shigella boydii: Global Burden of Infections

Journal: Science Journal of Clinical Medicine PDF  

Published: 09-Nov-15 Volume: 4 Issue: 6

DOI:10.11648/j.sjcm.20150406.12 ISSN: 2327-2724 (Print) 2327-2732 (online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Abstract

Phenotyping and Genotyping Characterization of Proteus vulgaris After Biofield Treatment

Journal: International Journal of Genetics and Genomics PDF  

Published: 09-Nov-15 Volume: 3 Issue: 6

DOI:10.11648/j.ijgg.20150306.12 ISSN: 2376-7340 (Print) 2376-7359 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Abstract

Characterization of Antimicrobial Susceptibility Profile of Biofield Treated Multidrug-resistant Klebsiella oxytoca

Journal: Applied Microbiology: Open Access PDF  

Published: 06-Oct-15 Volume: 1 Issue: 1

DOI:10.4172/2471-9315.1000101 ISSN: 2471-9315

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Harish Shettigar, Mayank Gangwar and Snehasis Jana

Abstract

Klebsiella are opportunistic pathogens that cause a wide spectrum of severe diseases. The aim of the present study was to investigate the impact of biofield treatment on multidrug resistant strain of K. oxytoca with respect to antibiogram pattern along with biochemical study and biotype number. Clinical lab isolate of K. oxytoca was divided into two groups i.e. control and treated. Control group remain untreated and treated group was subjected to Mr. Trivedi’s biofield. The analysis was done on day 10 after biofield treatment and compared with control group. Control and treated groups were analyzed for antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), biochemical reactions and biotype number using MicroScan Walk-Away® automated system. Experimental results showed the impact of biofield treatment on K. oxytoca and found alteration in both antimicrobial sensitivity and MIC values as compared with untreated group. Antimicrobial sensitivity of about 26.67% tested antimicrobials out of thirty was altered with respect to control. MIC results showed about 12.50% alterations in tested antimicrobials as compared to control. Biochemical study showed 24.24% alteration in tested biochemical reactions after biofield treatment. A significant change in biotype number (7713 5272) was identified after biofield treatment as compared to control (7775 4332). In treated group, a new species was identified as Kluyvera ascorbata, as compared to control, K. oxytoca. Study findings suggest that biofield treatment has a significant effect in altering the antimicrobial sensitivity, MIC values, biochemical reactions and biotype number of multidrug resistant strain of K. oxytoca. Biofield treatment could be applied to alter the antibiogram-resistogram pattern of antimicrobials.

Characterization of Physical, Thermal and Spectral Properties of Biofield Treated Date Palm Callus Initiation Medium

Journal: International Journal of Nutrition and Food Science PDF  

Published: 09-Nov-15 Volume: 4 Issue: 6

DOI:10.11648/j.ijnfs.20150406.20 ISSN: 2327-2694 (Print) 2327-2716 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Rakesh Kumar Mishra, Snehasis Jana

Abstract

The date palm is mainly cultivated for the production of sweet fruit. Date palm callus initiation medium (DPCIM) is used for plant tissue culture applications. The present work is intended to evaluate the impact of Mr. Trivedi’s biofield energy treatment on physical, thermal and spectral properties of the DPCIM. The control and treated DPCIM were evaluated by various analytical techniques such as X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, particle size analyzer (PSA), surface area analyzer and ultra violet-visible spectroscopy (UV-vis) analysis. The XRD analysis revealed a decrease in intensity of XRD peaks of the treated sample as compared to the control. The crystallite size of the treated DPCIM (81.02 nm) was decreased with respect to the control sample (84.99 nm). The DSC analysis showed a slight decrease in melting temperature of the treated sample. Additionally, the latent heat of fusion of treated sample was changed by 45.66% as compared to the control sample. The TGA analysis showed an increase in onset degradation temperature of the treated sample (182ºC) as compared to the control sample (142ºC). This indicated the increase in thermal stability of the treated DPCIM. PSA results demonstrated an increase in average particle size (d50) and size showed by 99% of particles (d99) by 19.2 and 40.4%, respectively as compared to the control sample. The surface area analyzer showed a decrease in surface area of treated DPCIM by 13.4%, which was well supported by the particle size results. UV spectra of the treated sample showed the disappearance of absorption peak 261 nm in treated sample as compared to the control. Overall, the result showed that biofield energy treatment has a paramount influence on physical, thermal and spectral properties of DPCIM. Therefore, it is assumed that biofield treated DPCIM could be used as a better medium for plant tissue culture applications.

Physicochemical and Spectroscopic Properties of Biofield Energy Treated Protose

Journal: American Journal of Biomedical and Life Sciences PDF  

Published: 09-Nov-15 Volume: 3 Issue: 6

DOI:10.11648/j.ajbls.20150306.11 ISSN: 2330-8818 (Print) 2330-880X (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Khemraj Bairwa, Snehasis Jana

Abstract

Protose is the enzyme digest of mixed proteins that is recommended for culture media, bulk production of enzymes, antibiotics, toxins, veterinary preparations, etc. This study was proposed to evaluate the effect of biofield energy treatment on the physicochemical and spectroscopic properties of protose. The study was achieved in two groups i.e. control and treated. The control group was remained as untreated, while the treated group was received Mr. Trivedi’s biofield energy treatment. Finally, both the control and treated samples were evaluated using various analytical techniques. The X-ray diffractograms (XRD) of control and treated samples showed the halo patterns peak that suggested the amorphous nature of both the samples of protose. The particle size analysis showed about 12.68% and 90.94 increase in the average particle size (d50) and d99 (particle size below which 99% particles are present) of treated protose with respect to the control. The surface area analysis revealed the 4.96% decrease in the surface area of treated sample as compared to the control sample. The differential scanning calorimetry (DSC) analysis revealed the 22.49% increase in the latent heat of fusion of treated sample as compared to the control. Thermogravimetric analysis (TGA) analysis showed increase in maximum thermal degradation temperature (Tmax) by 5.02% in treated sample as compared to the control. The increase in Tmax might be correlated with increased thermal stability of treated sample as compared to the control. Fourier transform infrared (FT-IR) study showed the alteration in the vibrational frequency of functional groups like N-H, C-H, and S=O of treated protose as compared to the control sample. Based on the overall analytical results, it is concluded that Mr. Trivedi’s biofield energy treatment has a significant impact on the physicochemical and spectral properties of protose. As a result, the treated protose might be more effective as a culture medium than the corresponding control.

Analysis of Genetic Diversity Using Simple Sequence Repeat (SSR) Markers and Growth Regulator Response in Biofield Treated Cotton (Gossypium hirsutum L.)

Journal: American Journal of Agriculture and Forestry PDF  

Published: 09-Nov-15 Volume: 3 Issue: 5

DOI:10.11648/j.ajaf.20150305.17 ISSN: 2330-8583 (Print) 2330-8591 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak , Mayank Gangwar, Snehasis Jana

Abstract

Cotton is the most important crop for the production of fiber that plays a key role in economic and social affairs. The aim of the study was to evaluate the impact of biofield energy treatment on cotton seeds regarding its growth, germination of seedling, glutathione (GSH) concentration, indole acetic acid (IAA) content and DNA fingerprinting using simple sequence repeat (SSR) markers for polymorphism analysis. The seeds of cotton cv. Stoneville-2 (Gossypium hirsutum L.) was obtained from DNA Land Marks Inc., Canada and divided into two groups. One group was remained as untreated, while the other was subjected to Mr. Trivedi biofield energy and referred as treated sample. The growth-germination of cotton seedling data showed higher germination (82%) in biofield treated seeds as compared to the control (68%). The alterations in length of shoot and root of cotton seedling was reported in the treated sample with respect to untreated seeds. However, the endogenous level of GSH in the leaves of treated cotton was increased by 27.68% as compared to the untreated sample, which may suggest an improved immunity of cotton plant. Further, the plant growth regulatory constituent i.e. IAA concentration was increased by 7.39%, as compared with the control. Besides, the DNA fingerprinting data, showed polymorphism (4%) between treated and untreated samples of cotton. The overall results suggest that the biofield energy treatment on cotton seeds, results in improved overall growth of plant, increase germination rate, GSH and IAA concentration were increased. The study assumed that biofield energy treatment on cotton seeds would be more useful for the production of cotton fiber.