Characterization of Physicochemical and Thermal Properties of Energy of Consciousness Healing Treated Copper (II) Chloride Using PXRD, DSC, and TGA/DTG

Journal: World Journal of Food Science and Technology  

Published: 14-Jul-17 Volume: 1 Issue: 2

DOI:10.11648/j.wjfst.20170102.15 ISSN: Print: 2637-6016 Online: 2637-6024

Authors: Alice Branton, Snehasis Jana

Abstract

Copper (II) chloride is an inorganic compound used as a source of copper ions for the treatment of several diseases like cancer, inflammation, hematological and neurological disorders. This article was aimed to evaluate the effect of The Trivedi Effect® - Energy of Consciousness Healing Treatment on the physicochemical and thermal properties of copper (II) chloride using PXRD, DSC, and TGA/DTG analysis. Copper chloride was divided into two parts, one part of copper chloride was considered as control (no Biofield Treatment was provided), while second part received The Trivedi Effect® - Biofield Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Alice Branton. The PXRD analysis showed that the relative intensities of the characteristic diffraction peak in Alice’s treated sample were significantly changed from -77.37% to 205.56% compared with the control sample. The crystallite size of the characteristic diffraction peak in the treated sample was altered from -14.29% to 40.01% compared with the control sample. The DSC analysis showed that the melting point of the treated copper chloride was significantly increased by 2.91% with a reduced latent heat of fusion (?H) by 28.73% compared to the control sample. The melting temperature of the 2nd peak in the treated sample was significantly increased by 7.08% with a significant enhancement of ?H by 77.37% compared with the control sample. The decomposition temperature of the treated sample was increased by 2.36% with a significant reduction of the enthalpy of decomposition by 18.46% compared with the control sample. The TGA analysis revealed the three steps of thermal degradation. The percentage weight loss in the treated sample was decreased by 0.47% and 9.63% in the 1st and 2nd steps of degradation, respectively, while the weight loss in the 3rd step was increased by 2.45% compared with the control sample. The total weight loss in the treated sample was increased by 1.32% compared with the control sample. The DTG analysis showed that the maximum thermal degradation temperature (Tmax) in the 1st, 2nd, and 3rd peaks of the Biofield Treated sample were increased by 1.20%, 2.00%, and 4.61% compared with the control sample. Briefly, The Trivedi Effect® - Energy of Consciousness Healing Treatment might produce a polymorphic form of copper (II) chloride that could be more stable during manufacturing, delivery or storage conditions than the untreated sample. The Biofield Energy Treated copper chloride would provide better therapeutic response against cancer, inflammation, wound healing, etc.

Evaluation of the Impact of Biofield Energy Healing Treatment (The Trivedi Effect®) on the Physicochemical, Thermal, Structural, and Behavioral Properties of Magnesium Gluconate

Journal: International Journal of Nutrition and Food Sciences PDF  

Published: 25-Feb-17 Volume: 6 Issue: 2

DOI:10.11648/j.ijnfs.20170602.13 ISSN: 2327-2694 (Print) 2327-2716 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Aileen Carol Lee, Aksana Hancharuk, Carola Marina Sand, Debra Jane Schnitzer, Rudina Thanasi, Eileen Mary Meagher, Faith Ann Pyka, Gary Richard Gerber, Johanna Catharina Stromsnas, Judith Marian Shapiro, Laura Nelson Streicher, Lorraine Marie Hachfeld, Matthew Charles Hornung, Patricia M. Rowe, Sally Jean Henderson, Sheila Maureen Benson, Shirley Theresa Holmlund, Stephen P. Salters, Parthasarathi Panda, Snehasis Jana

Abstract

Magnesium gluconate is a classical organometallic salt used for the prevention and treatment of magnesium deficiency diseases. The objective of the current research was to explore the influence of The Trivedi Effect® - Energy of Consciousness Healing Treatment on the physicochemical, thermal and behavioral properties of magnesium gluconate using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Magnesium gluconate was divided into two parts – one part was control without any Biofield Energy Treatment, while another part was treated with the Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The PXRD analysis exhibited significant alteration of the crystal morphology of the treated sample compared with the control sample. The crystallite size of the treated sample was remarkably changed from range -33.33% to 66.65% compared with the control sample. The average crystallite size was increased in the treated sample by 6.13% compared with the control sample. Particle size analysis revealed that the particle size at d10 value was significantly reduced in the treated sample by 13.20% compared with the control sample, although the particle size at d50 and d90 values were increased in the treated sample by 2.75% and 3.72%, respectively. The treated sample’s surface area was significantly enhanced (6.96%) compared with the control sample. The FT-IR and UV-vis analysis showed that the structure of magnesium gluconate remained similar in both the treated and control samples. The TGA analysis revealed that the weight loss of the first and second degradation steps in the treated sample was significantly decreased by 2.89% and 8.43%, respectively compared with the control sample, whereas at the third degradation step, the weight loss was enhanced by 14.80% compared with the control sample. The DSC analysis revealed that the melting point of the control and treated samples were 170.23°C and 170.25°C, respectively. The latent heat of fusion was significantly decreased by 6.15% in the treated sample compared with the control sample. The current study infers that The Trivedi Effect® - Energy of Consciousness Healing Treatment might lead to a new polymorphic form of magnesium gluconate, which could be more soluble and bioavailable compared with the untreated compound. Hence, the Biofield Energy Treated magnesium gluconate would be very useful to design better nutraceutical and/or pharmaceutical formulations that might offer better therapeutic responses against inflammatory diseases, immunological disorders, stress, aging and other chronic infections.

Structural and Natural Isotopic Abundance Analysis of Magnesium Gluconate Treated with Energy of Consciousness (The Trivedi Effect®) Using LC-MS and NMR

Journal: Chemical and Biomolecular Engineering PDF  

Published: 09-Mar-17 Volume: 2 Issue: 3

DOI:10.11648/j.cbe.20170203.11 ISSN: Not Available

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Aileen Carol Lee, Aksana Hancharuk, Carola Marina Sand, Debra Jane Schnitzer, Rudina Thanasi, Eileen Mary Meagher, Faith Ann Pyka, Gary Richard Gerber, Johanna Catharina Stromsnas, Judith Marian Shapiro, Laura Nelson Streicher, Lorraine Marie Hachfeld, Matthew Charles Hornung, Patricia M. Rowe, Sally Jean Henderson, Sheila Maureen Benson, Shirley Theresa Holmlund, Stephen P. Salters, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Abstract

The current research article was aimed to investigate the impact of The Trivedi Effect®- Energy of Consciousness Healing Treatment on the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) of magnesium gluconate using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® remotely by eighteen renowned Biofield Energy Healers and defined as the Trivedi Effect® Treated sample. The liquid chromatogram of the control sample showed two peaks at Rt of 1.81 and 2.06 min, whereas the Trivedi Effect® Treated sample displayed these peaks at Rt of 1.79 and 2.03 min. The ESI-MS spectra of the control and the Trivedi Effect® Treated samples revealed the presence of the mass for magnesium gluconate ion in two forms at m/z 447 (adduct form with methanol) and 415 (protonated ion) in positive ionization mode. But, it showed the mass for the gluconate ion at m/z 195 in the negative ionization mode. The fragmentation pattern of magnesium gluconate in the treated sample was notably altered as compared to the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) in two magnesium gluconate ion forms at m/z 447 and 415 in treated sample was significantly decreased by 59.82% and 55.44%, respectively compared with the control sample. The percentage change in the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) was remarkably decreased in the magnesium gluconate ion at m/z 447 in the treated sample by 78.26% compared with the control sample. Consequently, the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O) in gluconate ion in the treated sample was significantly increased by 37.35% with respect to the control sample. Thus, the Trivedi Effect® treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergies, aging, inflammatory diseases, immunological disorders, and other chronic infections. The treated magnesium gluconate might be helpful to design the novel potent enzyme inhibitors by using its kinetic isotope effects.

A Comprehensive Analytical Evaluation of the Trivedi Effect® – Energy of Consciousness Healing Treatment on the Physical, Structural, and Thermal Properties of Zinc Chloride

Journal: American Journal of Applied Chemistry PDF  

Published: 25-Feb-17 Volume: 5 Issue: 1

DOI:10.11648/j.ajac.20170501.12 ISSN: 2330-8753 (Print) 2330-8745 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Aileen Carol Lee, Aksana Hancharuk, Carola Marina Sand, Debra Jane Schnitzer, Rudina Thanasi, Eileen Mary Meagher, Faith Ann Pyka, Gary Richard Gerber, Johanna Catharina Stromsnas, Judith Marian Shapiro, Laura Nelson Streicher, Lorraine Marie Hachfeld, Matthew Charles Hornung, Patricia M. Rowe, Sally Jean Henderson, Sheila Maureen Benson, Shirley Theresa Holmlund, Stephen P. Salters, Parthasarathi Panda, Snehasis Jana

Abstract

Zinc chloride has an importance in pharmaceutical and nutraceutical industries for the prevention and treatment of several diseases. The objective of the current study was to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Treatment) on physical, structural, and thermal properties of zinc chloride using PXRD, PSD, FT-IR, UV-vis, TGA, and DSC analysis. Zinc chloride was divided into two parts – one part was control, while other part was treated with The Trivedi Effect® remotely by eighteen renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. A significant change of the crystallite size and relative intensities of the PXRD peaks was observed in The Trivedi Effect® treated sample with respect to the control sample. The average crystallite size of the treated sample was significantly increased by 41.22% compared with the control sample. The size of the particles at d10, d50, and d90 was increased by 8.33%, 6.27%, and 1.83%, respectively in the treated sample compared with the control sample. The surface area of the treated sample was decreased by 5.90% compared with the control sample. The FT-IR spectroscopic analysis revealed that Zn-Cl stretching in the control and treated sample were at 511 and 507 cm-1, respectively. The UV-vis analysis exhibited that wavelength of the maximum absorbance of both the control and treated samples were at 196.4 and 196.3 nm, respectively. The TGA analysis exhibited that weight loss of the treated sample was reduced by 13.98% and 2.43% in the 1st and 2nd steps of degradation, respectively. Consequently, the DSC analysis revealed that the maximum thermal decomposition temperature of the treated sample (489.06°C) was significantly increased by 15.75% compared with the control sample (422.50°C). Moreover, a significant increase in the enthalpy of decomposition was noticed in the treated sample (?Hdecomp = 131.40 J/g) by 137.10% compared with the control sample (?Hdecomp = 55.42 J/g). The current study anticipated that The Trivedi Effect® Treatment might lead to produce a new polymorphic form of zinc chloride, which could have better powder flowability and thermal stability. The treated sample could be more stable during manufacturing, delivery or storage conditions than the untreated sample. Hence, the treated zinc chloride would be very useful to design better nutraceutical and/or pharmaceutical formulations that might offer better therapeutic response against inflammatory diseases, immunological disorders, aging, stress, cancer, etc.

Evaluation of the Trivedi Effect®- Energy of Consciousness Energy Healing Treatment on the Physical, Spectral, and Thermal Properties of Zinc Chloride

Journal: American Journal of Life Sciences PDF  

Published: 25-Feb-17 Volume: 5 Issue: 1

DOI:10.11648/j.ajls.20170501.13 ISSN: 2328-5702 (Print) 2328-5737 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Cathryn Dawn Nykvist, Celine Lavelle, Daniel Paul Przybylski, Dianne Heather Vincent, Dorothy Felger, Douglas Jay Konersman, Elizabeth Ann Feeney, Jay Anthony Prague, Joanne Lydia Starodub, Karan Rasdan, Karen Mie Strassman, Leonid Soboleff, Maire Anne Mayne, Mary M. Keesee, Padmanabha Narayana Pillai, Pamela Clarkson Ansley, Ronald David Schmitz, Sharyn Marie Sodomora, Kalyan Kumar Sethi, Parthasarathi Panda, Snehasis Jana

Abstract

Zinc chloride has the importance in pharmaceutical/nutraceutical industries for the prevention and treatment of several diseases. The objective of the current study was to investigate the impact of The Trivedi Effect®-Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on physical, structural, and thermal properties of zinc chloride using PXRD, PSD, FT-IR, UV-vis, TGA, and DSC analysis. Zinc chloride was divided into two parts. One part was denoted as the control without any, while the other part was defined as the Trivedi Effect® Treated sample, which received the Trivedi Effect® Treatment remotely from eighteen renowned Biofield Energy Healers. The PXRD analysis revealed that the crystallite size and relative intensities of the PXRD peaks significantly altered in the treated sample compared with the control sample. The crystallite size of treated sample was decreased by 4.19% compared with the control sample. The particle size at d10 and d50 of the Biofield Energy Treated sample decreased by 4.72% and 2.70%, respectively compared with the control sample. But, the particle size of the treated sample increased at d90 by 0.83 compared with the control sample. Consequently, the surface area was increased by 3.22% in the treated sample compared with the control sample. The FT-IR spectroscopic analysis revealed that Zn-Cl stretching in the control and treated sample was at 520 cm-1 and 521 cm-1, respectively. The UV-vis analysis exhibited that the wavelength of the maximum absorbance of the control and treated samples was at 196.4 and 196.2 nm, respectively. The TGA thermograms revealed two steps of the thermal degradation and the weight loss of the treated sample was significantly reduced by 22.54% in the 1st step of degradation compared with the control sample. The DSC analysis showed that the enthalpy of decomposition was significantly increased by 34.9% in the treated sample (89.17 J/g) compared with the control sample (66.10 J/g). Overall, DSC and TGA analysis indicated that the thermal stability of the treated sample was increased compared with the control sample. The current study anticipated that The Trivedi Effect®-Energy of Consciousness Healing Treatment might lead to generate a new polymorphic form of zinc chloride, which would be more soluble, stable, and higher absorption rate compared with the control sample. Hence, the treated zinc chloride could be very useful to design the various forms of nutraceuticals and pharmaceutical formulation which might be providing a better therapeutic response against inflammatory diseases, immunological disorders, aging, stress, cancer, etc.

Evaluation of Isotopic Abundance Ratio and Structural Properties of Magnesium Gluconate After Treatment with the Energy of Consciousness Using LC-MS and NMR Spectroscopy

Journal: Advances in Bioscience and Bioengineering PDF  

Published: 25-Feb-17 Volume: 5 Issue: 1

DOI:10.11648/j.abb.20170501.11 ISSN: 2330-4154 (Print) 2330-4162 (Online)

Authors: Mahendra Kumar Trivedi , Alice Branton , Dahryn Trivedi , Gopal Nayak , Cathryn Dawn Nykvist , Celine Lavelle , Daniel Paul Przybylski , Dianne Heather Vincent , Dorothy Felger , Douglas Jay Konersman , Elizabeth Ann Feeney , Jay Anthony Prague , Joanne Lydia Starodub , Karan Rasdan , Karen Mie Strassman , Leonid Soboleff , Maire Anne Mayne , Mary M. Keesee , Padmanabha Narayana Pillai , Pamela Clarkson Ansley , Ronald David Schmitz , Sharyn Marie Sodomora , Kalyan Kumar Sethi , Parthasarathi Panda , Snehasis Jana

Abstract

The current research work was aimed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing) on the structural properties and isotopic abundance ratio (PM+1/PM) of magnesium gluconate using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts. One part was denoted as the control, while the another part was defined as The Trivedi Effect® Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The total ion chromatogram of the control sample showed two peaks at Rt of 1.81 and 2.06 min, whereas the treated sample displayed peaks at Rt of 1.79 and 2.04 min. The ESI-MS spectra of the control and the treated samples revealed the presence of the mass for magnesium gluconate ion in two forms at m/z 447 (adduct form with methanol) and 415 (protonated ion) in positive ionization mode. But, it showed the mass for the gluconate ion at m/z 195 in the negative ionization mode. The fragmentation pattern of magnesium gluconate in the treated sample was notably altered compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and treated samples. The LC-MS based isotopic abundance ratio analysis indicated that the PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) in the treated magnesium gluconate ion at m/z 415 was significantly decreased by 79.24% compared with the control sample. Similarly, the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O) in the gluconate ion was decreased by 6.62% in the treated sample compared with the control sample. The treated magnesium gluconate might be beneficial in the nutraceutical and/or pharmaceutical industries for designing various forms of formulations, which could be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. Consequently, The Trivedi Effect® - Energy of Consciousness Healing Treated magnesium gluconate would be more helpful for understanding the enzymatic reactions as well as assist in the designing of the novel potent enzyme inhibitors by applying its kinetic isotope effects.

Physicochemical, Thermal, Structural, and Behavioral Properties Analysis in Magnesium Gluconate: An Effect of the Energy of Consciousness (The Trivedi Effect®)

Journal: International Journal of Biomedical Materials Research PDF  

Published: 09-Mar-17 Volume: 5 Issue: 2

DOI:10.11648/j.ijbmr.20170502.11 ISSN: 2330-7569 (Print) 2330-7579 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Cathryn Dawn Nykvist, Celine Lavelle, Daniel Paul Przybylski, Dianne Heather Vincent, Dorothy Felger, Douglas Jay Konersman, Elizabeth Ann Feeney, Jay Anthony Prague, Joanne Lydia Starodub, Karan Rasdan, Karen Mie Strassman, Leonid Soboleff, Maire Anne Mayne, Mary M. Keesee, Padmanabha Narayana Pillai, Pamela Clarkson Ansley, Ronald David Schmitz, Sharyn Marie Sodomora, Kalyan Kumar Sethi, Parthasarathi Panda, Snehasis Jana

Abstract

Magnesium gluconate is an organometallic pharmaceutical/nutraceutical used for the prevention and treatment of various diseases caused by the low level of magnesium. The aim of the present study was to investigate the influence of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing) on the physicochemical, thermal, structural, and behavioral properties of magnesium gluconate using powder PXRD, PSD, FT-IR, UV-visible, TGA, and DSC analysis. Magnesium gluconate was divided into two parts - one part was denoted as the control, while the another part was treated with The Trivedi Effect® remotely by eighteen renowned Biofield Energy Healers and defined as the Trivedi Effect® Treated sample. The PXRD analysis exhibited the significant alteration of the crystal morphology of the treated sample compared with the control sample. The crystallite size of the treated sample was significantly altered from -39.99% to 62.57% compared with the control sample. The average crystallite size of the treated sample was decreased by 9.71% compared with the control sample. Particle size of the treated sample at d10, d50, and d90 value was significantly increased by 5.36%, 23.10% and 11.11%, respectively compared with the control sample. The surface area of the treated sample was significantly decreased by 9.76% compared to the control sample. The FT-IR and UV-vis analysis showed that the structural characteristic of the magnesium gluconate remained same in the treated sample compared with control sample. The TGA data revealed that the weight loss of the treated sample in the first and third steps of degradation was increased by 31.58% and 5.94%, respectively, whereas in the second step of degradation, the weight loss was decreased by 7.57% compared with the control sample. The DSC analysis showed that the melting point of the control and treated samples were at 170.29°C and 169.76°C, respectively. The latent heat of fusion of the treated sample was increased by 4.18% compared with the control sample. The current study evaluated that The Trivedi Effect® - Energy of Consciousness Healing Treatment might lead to a new polymorphic form of the magnesium gluconate, which could be more soluble, powder flowability and long-term storage stability compared with the control sample. Hence, the Trivedi Effect® Treated magnesium gluconate would be very useful to design better nutraceutical and/or pharmaceutical formulations that might provide better therapeutic response against inflammatory diseases, immunological disorders, and other chronic infections.

Evaluation of Structural Properties and Isotopic Abundance Ratio of Biofield Energy Treated (The Trivedi Effect®) Magnesium Gluconate Using LC-MS and NMR

Journal: European Journal of Biophysics PDF  

Published: 25-Feb-17 Volume: 5 Issue: 1

DOI:10.11648/j.ejb.20170501.12 ISSN: 2329-1745 (Print) 2329-1737 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Alan Joseph Balmer, Dimitrius Anagnos, Janice Patricia Kinney, Joni Marie Holling, Joy Angevin Balmer, Lauree Ann Duprey-Reed, Vaibhav Rajan Parulkar, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Abstract

The current research work was designed to explore the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) by using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect®- Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The LC-MS analysis of the both control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.53 min with similar fragmentation pattern. The relative peak intensities of the fragment ions of the treated sample were significantly changed compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) in the treated sample was significantly increased by 34.33% compared with the control sample. Subsequently, the percentage change of the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) was significantly decreased in the treated sample by 64.08% as compared to the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly changed compared with the control sample. Thus, the treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. The Biofield Energy Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors by using its kinetic isotope effects.

Evaluation of Physicochemical, Thermal, Structural, and Behavioral Properties of Magnesium Gluconate Treated with Energy of Consciousness (The Trivedi Effect®)

Journal: Journal of Drug Design and Medicinal Chemistry PDF  

Published: 14-Mar-17 Volume: 3 Issue: 1

DOI:10.11648/j.jddmc.20170301.12 ISSN: 2472-355X (Print) 2472-3576 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Alan Joseph Balmer, Dimitrius Anagnos, Janice Patricia Kinney, Joni Marie Holling, Joy Angevin Balmer, Lauree Ann Duprey-Reed, Vaibhav Rajan Parulkar, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Abstract

Magnesium gluconate is a classical organometallic salt used for the prevention and treatment of magnesium deficiency diseases. The objective of the current research was to explore the influence of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the physicochemical, structural, thermal and behavioral properties using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Magnesium gluconate was divided into two parts – one part was control, while another part was treated with The Trivedi Effect® - Energy of Consciousness Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The PXRD analysis exhibited significant alteration of the crystal morphology of the treated sample compared with the control sample. The crystallite size of the treated sample was remarkably changed from range -69.99% to 71.40% compared with the control sample. The average crystallite size was significantly decreased in the treated sample by 13.61% compared with the control sample. Particle size analysis revealed that the particle size in the treated sample at d10, d50, and d90 value was significantly decreased by 5.19%, 26.77%, and 18.22%, respectively compared with the control sample. The treated sample’s surface area was significantly enhanced (12.82%) compared with the control sample. The FT-IR and UV-vis analysis showed that the structure of the magnesium gluconate remained the same in both the treated and control samples. The TGA analysis revealed the four steps thermal degradation of the both samples and the total weight loss of the Biofield Energy Treated sample was increased by 0.55% compared with the control sample. The DSC analysis revealed that the melting temperature of the treated sample (171.72°C) was increased by 0.21% compared with the control sample (171.36°C). The latent heat of fusion was increased by 4.66% in the treated sample compared with the control sample. This result indicated that the thermal stability of treated sample was improved compared with the control sample. The current study infers that The Trivedi Effect® - Biofield Energy Healing might lead to a new polymorphic form of magnesium gluconate, which would be more soluble, bioavailable, and thermally stable compared with the untreated compound. Hence, the treated magnesium gluconate would be very useful to design better nutraceutical/pharmaceutical formulations that might offer better therapeutic responses against inflammatory diseases, immunological disorders, stress, aging and other chronic infections.

Investigation of Physicochemical, Spectral, and Thermal Properties of Sodium Selenate Treated with the Energy of Consciousness (The Trivedi Effect®)

Journal: American Journal of Life Sciences PDF  

Published: 07-Mar-17 Volume: 5 Issue: 1

DOI:10.11648/j.ajls.20170501.15 ISSN: 2328-5702 (Print) 2328-5737 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Aileen Carol Lee, Aksana Hancharuk, Carola Marina Sand, Debra Jane Schnitzer, Rudina Thanasi, Eileen Mary Meagher, Faith Ann Pyka, Gary Richard Gerber, Johanna Catharina Stromsnas, Judith Marian Shapiro, Laura Nelson Streicher, Lorraine Marie Hachfeld, Matthew Charles Hornung, Patricia M. Rowe, Sally Jean Henderson, Sheila Maureen Benson, Shirley Theresa Holmlund, Stephen P. Salters, Parthasarathi Panda, Snehasis Jana

Abstract

Sodium selenate is used for the prevention and treatment of various disorders like cancer, diabetes, inflammation diseases, etc. The objective of the current study was to evaluate the effect of The Trivedi Effect®-Energy of Consciousness Healing Treatment (Biofield Energy Treatment) on physicochemical, spectral, and thermal properties of The Trivedi Effect®-Biofield Energy Treated sodium selenate using various analytical methods such as PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Sodium selenate was divided into two parts – one part was control without any treatment and another part was treated with The Trivedi Effect®-Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers and defined as The Trivedi Effect®-Energy of Consciousness Healing Treated sample. The PXRD analysis showed the significant alteration of the crystallite size of the treated sample in the range of -36.36% to 133.24% as compared to the control sample. However, the average crystallite size of the treated sample was significantly decreased by 8.31% as compared to the control sample. Overall, the crystal morphology of the treated sample was different from the control sample. The particle sizes d10, d50, and d90 values of the treated sample were significantly decreased by 3.40%, 9.75%, and 8.52%, respectively as compared to the control sample. Consequently, the surface area of the treated sample was significantly increased by 5.26% from the control sample. Both the control and treated FT-IR spectra indicated the presence of sharp and strong absorption bands at 887 cm-1 due to the Se=O stretching. The UV-vis spectroscopic analysis displayed that the wavelength for the maximum absorbance of both the control and treated samples were at 204.1 and 204.0 nm, respectively. TGA analysis revealed that the total weight loss of the treated sample was reduced by 1.24% as compared with the control sample. The DSC analysis showed that the treated sample (588.77°C) had higher melting point than the control sample (588.60°C). The latent heat of fusion was reduced in the treated sample by 8.01% compared to the control sample. Thus, The Trivedi Effect®-Biofield Energy Healing might lead to generate a new polymorphic (enantiotropic) form of sodium selenate which would be more soluble, bioavailable, and thermally stable compared with the untreated sample. The Trivedi Effect treated sodium selenate would be very useful to design better nutraceutical and/or pharmaceutical formulations that might offer better therapeutic response against inflammatory diseases, immunological disorders, stress, aging, infectious diseases, cancer, diabetes, heart diseases, Alzheimer’s disease, etc.

Impact of Consciousness Energy Healing Treatment (The Trivedi Effect®) on Physical, Spectroscopic, and Thermal Properties of Withania somnifera (Ashwagandha) Root Extract

Journal: International Journal of Food Science and Biotechnology PDF  

Published: 09-Mar-17 Volume: 2 Issue: 1

DOI:10.11648/j.ijfsb.20170201.12 ISSN: Not Available

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Aileen Carol Lee, Aksana Hancharuk, Carola Marina Sand, Debra Jane Schnitzer, Rudina Thanasi, Eileen Mary Meagher, Faith Ann Pyka, Gary Richard Gerber, Johanna Catharina Stromsnas, Judith Marian Shapiro, Laura Nelson Streicher, Lorraine Marie Hachfeld, Matthew Charles Hornung, Patricia M. Rowe, Sally Jean Henderson, Sheila Maureen Benson, Shirley Theresa Holmlund, Stephen P. Salters, Parthasarathi Panda, Snehasis Jana

Abstract

Withania somnifera (Ashwagandha) root extract and their active metabolites possess a broad range of pharmacological activities. The current study aimed to explore the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing) on the physical, spectroscopic, and thermal properties of ashwagandha root extract using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Ashwagandha root extract was divided into two parts – one part was control without any and another part was treated with The Trivedi Effect® - Energy of Consciousness Healing Treatment remotely by eighteen renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. The PXRD analysis exhibited that both the treated and control samples were amorphous in nature. The particle sizes at d10, d50, and d90 values of the treated sample were significantly decreased by 12.23%, 10.97%, and 1.25%, respectively with respect to the control sample. The surface area analysis revealed that the surface area of the treated sample was significantly increased by 14.29% as compared to the control sample. The FT-IR analysis indicated the alteration of the force constant for the functional groups of the treated sample in comparison to the control sample. The UV-vis analysis revealed that the wavelength for the maximum absorbance of the control and treated samples were at 205.8 and 206.0 nm, respectively. The TGA analysis revealed that the total weight loss was decreased by 0.70% in the treated sample as compared to the control sample. This indicated that the treated sample was thermally more stable as compared to the control sample. The DSC analysis indicated that the melting point of the treated sample (190.48°C) was decreased by 4.91% than the control sample (203.45°C). The latent heat of fusion of the treated (4.94 J/g) sample was significantly decreased by 42.96% compared with the control (8.66 J/g) sample. The current findings suggest that The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing) might have the astounding capacity to enhance the solubility, absorption, dissolution, and finally, bioavailability of ashwagandha root extract in various form of pharmaceutical and nutraceutical formulation by modifying its particle size and surface area. Thus, the treated ashwagandha root extract might provide better therapeutic response against against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.

LC-MS, GC-MS, and NMR Spectroscopic Analysis of Withania somnifera (Ashwagandha) Root Extract After Treatment with the Energy of Consciousness (The Trivedi Effect®)

Journal: European Journal of Biophysics PDF  

Published: 09-Mar-17 Volume: 5 Issue: 2

DOI:10.11648/j.ejb.20170502.11 ISSN: 2329-1745 (Print) 2329-1737 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Aileen Carol Lee, Aksana Hancharuk, Carola Marina Sand, Debra Jane Schnitzer, Rudina Thanasi, Eileen Mary Meagher, Faith Ann Pyka, Gary Richard Gerber, Johanna Catharina Stromsnas, Judith Marian Shapiro, Laura Nelson Streicher, Lorraine Marie Hachfeld, Matthew Charles Hornung, Patricia M. Rowe, Sally Jean Henderson, Sheila Maureen Benson, Shirley Theresa Holmlund, Stephen P. Salters, Parthasarathi Panda, Snehasis Jana

Abstract

Withania somnifera (Ashwagandha) root extract is very popular ancient herbal medicine. The current study was designed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment on the structural characterization of the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control, without any Biofield Energy Healing Treatment, while another part was treated with the Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The retention time of the phytoconstituents remained same in both the control and treated samples, whereas the peak area% at respective retention time was significantly altered. The peak area% of the treated sample at Rt of 5.35, 5.55, 5.94, 6.25, 6.63, 6.76, 7.92, 8.04, 8.60, 8.73, and 9.31 min were significantly reduced by 6.15% to 60.67% compared with the control sample at Rt of 5.43, 5.65, 5.95, 6.29, 6.76, 6.85, 8.03, 8.14, 8.68, 8.78, and 9.30 min. Consequently, the peak area% of the treated sample at Rt of 7.25, 7.30, 8.27, and 8.47 min were significantly increased by 26.32%, 7.99%, 16.93% and 7.97% compared with the control sample at Rt of 7.37, 7.41, 8.36, and 8.55 min, respectively. A total of 13 withanolides were proposed with their structure from the deduced molecular mass at m/z 470, 472, 488, 504, 782, and 991 through LC-MS, GC-MS, 1H and 13C NMR analysis of both the control and treated samples. Viscosa lactone B, 27-hydroxy withanolide A, (20S, 22R)-?, 6?-epoxy-?, 5?, 27-trihydroxy-1-oxowitha-24-enolide, (20S, 22R)-4?, 5?, 6?, 27-tetrahydroxy-1-oxo-with-2, 24-dienolide were proposed in the control and treated samples at Rt of 6.85 and 7.30 min, respectively. Dihydrowithanolide D was only identified in the control sample at Rt of 7.41 min, whereas withanoside IV or withanoside VI was only present in the Biofield Treated sample at Rt of 6.76 min. Withanolide A, withaferin A, withanone, withanolide D, ixocarpalactone A and withanolide sulfoxide were found in both the control and treated samples. These findings suggest that The Trivedi Effect® - Energy of Consciousness Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of W. somnifera extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.

Evaluation of the Physicochemical, Spectral, and Thermal Properties of Sodium Selenate Treated with the Energy of Consciousness (The Trivedi Effect®)

Journal: Advances in Bioscience and Bioengineering PDF  

Published: 07-Mar-17 Volume: 5 Issue: 1

DOI:10.11648/j.abb.20170501.12 ISSN: 2330-4154 (Print) 2330-4162 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Cathryn Dawn Nykvist, Celine Lavelle, Daniel Paul Przybylski, Dianne Heather Vincent, Dorothy Felger, Douglas Jay Konersman, Elizabeth Ann Feeney, Jay Anthony Prague, Joanne Lydia Starodub, Karan Rasdan, Karen Mie Strassman, Leonid Soboleff, Maire Anne Mayne, Mary M. Keesee, Padmanabha Narayana Pillai, Pamela Clarkson Ansley, Ronald David Schmitz, Sharyn Marie Sodomora, Kalyan Kumar Sethi, Parthasarathi Panda, Snehasis Jana

Abstract

Sodium selenate is a common ingredient in nutraceuticals/pharmaceuticals as a source of selenium. The objective of the current study was to evaluate the impact of The Trivedi Effect® - Energy of Consciousness (Biofield Energy Healing) on the physicochemical, spectral, and thermal properties of The Trivedi Effect® Treated sodium selenate using various analytical methods such as PXRD, PSD, FT-IR, UV-vis, DSC, and TGA. Sodium selenate was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received The Trivedi Effect® Treatment remotely from eighteen renowned Biofield Energy Healers. The PXRD analysis of the treated sample showed the significant alteration of the crystallite size in the range of -16.63% to 42.9% compared with the control sample. However, the average crystallite size of the treated sodium selenate was increased by 1.01% compared with the control sample. The particle sizes d10, d50, and d90 values of the treated sample were significantly decreased by 6.14%, 16.56%, and 18.82%, respectively compared with the control sample. Consequently, the surface area of the treated sample was significantly increased by 10.52% compared with the control sample. Both control and treated FT-IR spectra indicated the presence of sharp and strong absorption bands at 888 and 887 cm-1, respectively due to the Se=O stretching. The UV-vis spectroscopic analysis displayed that the wavelength for the maximum absorbance of the control and treated sodium selenate were at 204.8 and 204.9 nm, respectively. The TGA of the control and treated samples exhibited two steps of thermal degradation, and the total weight loss was slightly increased by 1.16% in the treated sample compared with the control sample. The DSC thermograms showed the latent heat of fusion (?H) was significantly elevated by 6.37% in the treated sample compared with the control sample. The current study revealed that The Trivedi Effect® - Energy of Consciousness (Biofield Energy Healing) might generate a new polymorphic form of sodium selenate which could be more soluble, thermally more stable, and higher absorption rate compared with the untreated sample. Hence, The Trivedi Effect® treated sodium selenate would be very useful to design the various forms of nutraceuticals and/or pharmaceutical formulation which might be providing a better therapeutic response against heart disease (i. e. Atherosclerosis), viral diseases (i. e. Hepatitis), infectious diseases (i. e. Dermatitis, Psoriasis), Parkinson’s Disease, Alzheimer’s Disease, etc.

A Comprehensive Physical, Spectroscopic, and Thermal Characterization of Withania somnifera (Ashwagandha) Root Extract Treated with the Energy of Consciousness (The Trivedi Effect®)

Journal: International Journal of Biomedical Materials Research PDF  

Published: 07-Mar-17 Volume: 5 Issue: 1

DOI:10.11648/j.ijbmr.20170501.12 ISSN: 2330-7560 (Print) 2330-7579 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Cathryn Dawn Nykvist, Celine Lavelle, Daniel Paul Przybylski, Dianne Heather Vincent, Dorothy Felger, Douglas Jay Konersman, Elizabeth Ann Feeney, Jay Anthony Prague, Joanne Lydia Starodub, Karan Rasdan, Karen Mie Strassman, Leonid Soboleff, Maire Anne Mayne, Mary M. Keesee, Padmanabha Narayana Pillai, Pamela Clarkson Ansley, Ronald David Schmitz, Sharyn Marie Sodomora, Kalyan Kumar Sethi, Parthasarathi Panda, Snehasis Jana

Abstract

Withania somnifera (Ashwagandha) root extract possesses a broad range of pharmacological activities. The aim of current study was to explore the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on the physical, spectroscopic, and thermal properties of ashwagandha root extract using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received The Trivedi Effect® Treatment remotely from eighteen renowned Biofield Energy Healers. The PXRD analysis exhibited that both the control and The Trivedi Effect® - Biofield Energy Treated samples were amorphous in nature. The particle size values at d10 and d50 of the treated sample were decreased by 4.66% and 6.02% and at d90 slightly increased by 0.99% compared with the control sample. The average surface area of the treated (0.130 m2/g) sample was increased by 4.84% compared with the control (0.124 m2/g) sample. The FT-IR results suggested that the force constant of O-H (stretching), and C-O (stretching) functional groups of the treated sample were reduced compared with the control sample. The UV-vis analysis revealed that the wavelength for the maximum absorbance of the control and treated samples were at 205.4 nm and 205.6 nm, respectively. The TGA thermograms exhibited three steps of the degradation process, and the total weight loss of the treated (86.45%) sample was increased by 1.44% compared with the control (85.22%) sample. The DSC analysis revealed that melting point of the treated (201.67°C) sample was reduced by 0.87% compared with the control (203.43°C) sample. The latent heat of fusion of the treated (6.77 J/g) sample was significantly decreased by 21.82% compared to the control (8.67 J/g) sample. Hence, the treated sample would be more advantageous for the better therapeutic responses compared to the control sample. These findings suggest that The Trivedi Effect® - Energy of Consciousness Healing Treatment might have the astounding capacity to enhance the solubility, dissolution, absorption, and bioavailability of ashwagandha root extract in various forms of pharmaceutical/nutraceutical formulations by modifying its particle size and surface area. Thus, The Trivedi Effect® - Energy of Consciousness Healing Treated ashwagandha root extract might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.

Liquid Chromatography – Mass Spectrometry (LC-MS) Analysis of Withania somnifera (Ashwagandha) Root Extract Treated with the Energy of Consciousness

Journal: American Journal of Quantum Chemistry and Molecular Spectroscopy PDF  

Published: 21-Mar-17 Volume: 2 Issue: 1

DOI:10.11648/j.ajqcms.20170201.11 ISSN: Not Available

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Cathryn Dawn Nykvist, Celine Lavelle, Daniel Paul Przybylski, Dianne Heather Vincent, Dorothy Felger, Douglas Jay Konersman, Elizabeth Ann Feeney, Jay Anthony Prague, Joanne Lydia Starodub, Karan Rasdan, Karen Mie Strassman, Leonid Soboleff, Maire Anne Mayne, Mary M. Keesee, Padmanabha Narayana Pillai, Pamela Clarkson Ansley, Ronald David Schmitz, Sharyn Marie Sodomora, Kalyan Kumar Sethi, Parthasarathi Panda, Snehasis Jana

Abstract

Withania somnifera (ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect®-Biofield Energy Healing Treatment (Energy of Consciousness) on phytoconstituents present in the ashwagandha root extract using LC-MS. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The LC-MS analysis of the control and treated samples showed a very close retention time (Rt), indicated that the polarity of the phytoconstituents present in the root extract are same. The numbers of peaks observed in the total ion chromatograms were 28 and 29 in the control and treated samples, respectively. The change in the peak height% of the phytoconstituents in the treated sample was altered significantly within the range of -50.91% to 118.12% compared with the control sample. Similarly, the change in the peak area% of most of the phytoconstituents in the treated ashwagandha was significantly altered within the range of -54.95% to 66.95% compared with the control sample. An additional peak was appeared in the treated sample at Rt of 5.72 minutes, which was not found in the control sample. The LC-MS spectra indicated the presence of possible withanolides like ?-hydroxy-2,3-dihydro-withanolide F, withanolide A, withaferine A, withanone, withanolide D, ixocarpalactone A, withanolide S, thiowithanolide, etc. in both the samples. The peak are percentage (%) was altered in the identified withanolides, but withanolide sulfoxide was increased significantly by 12.44% in the treated sample compared with the control sample. These results indicated that The Trivedi Effect® - Biofield Energy Treatment might have an impact on the intrinsic physicochemical properties of the phytoconstituents present in the ashwagandha root extract. This could be the probable cause of alteration in the peak height, peak area, and appearance of a new peak in the treated sample. As a result, the concentrations of the phytoconstituents altered in the treated sample compared with the control sample. The treated ashwagandha root extract would be helpful for designing better pharmaceutical/nutraceutical formulations which might be providing a better therapeutic response against autoimmune diseases, nervous and sexual disorders, infectious diseases, antiaging, diabetes, cancer, ulcer, immunological disorders, stress, arthritis, etc.

Effect of the Energy of Consciousness (The Trivedi Effect®) on Withania somnifera Root Extract Using Gas Chromatography – Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy

Journal: Journal of Diseases and Medicinal Plants PDF  

Published: 14-Mar-17 Volume: 3 Issue: 2

DOI:10.11648/j.jdmp.20170302.11 ISSN: 2469-8202 (Print) 2469-8210 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Cathryn Dawn Nykvist, Celine Lavelle, Daniel Paul Przybylski, Dianne Heather Vincent, Dorothy Felger, Douglas Jay Konersman, Elizabeth Ann Feeney, Jay Anthony Prague, Joanne Lydia Starodub, Karan Rasdan, Karen Mie Strassman, Leonid Soboleff, Maire Anne Mayne, Mary M. Keesee, Padmanabha Narayana Pillai, Pamela Clarkson Ansley, Ronald David Schmitz, Sharyn Marie Sodomora, Kalyan Kumar Sethi, Parthasarathi Panda, Snehasis Jana

Abstract

Withania somnifera (Ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing) on phytoconstituents present in the ashwagandha root extract using GC-MS and NMR. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received The Trivedi Effect® - Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The GC-MS data indicated that the peak height and peak area of The Trivedi Effect® treated sample were found to be altered compared with the control sample. The peak height of the phytoconstituents present in the treated ashwagandha sample was altered significantly in the range of -8.32% to 89.25% compared with the control sample. Similarly, the peak area of the treated sample was altered significantly in the range of - 4.28% to 216.30% compared with the control sample. Overall, the change in the peak area% of the treated sample was significantly altered in the range of -18.29% to 170.18% compared with the control sample. The GC-MS and NMR analysis results identified the presence of withanolides such as glyco-withanolides, alkaloids, and sugars in the root extract in both the sample. The peak area of 2,3,4,5-tetrahydropyridazine (1), methyl ethyl sulfoxide (2), 5,6-dihydro-2-methyl-4(H)pyran-3,4- dione (4), diethoxy-2-methyl-propane (5), 2,3,4,5-tetrahydroxy-tetrahydro-pyran (6), and 3,4-dimethyl-2(3H)-furanone (7) were significantly increased by 170.18%, 58.21%, 7.74%, 139.50%, 23.16%, and 45.63%, respectively in the treated sample compared with the control sample. On the contrary, the peak area% of 2-hydroxy-?-butyrolactone (3) was decreased by - 14.96% in the treated ashwagandha compared with the control sample. From the results, it can be hypothesized that The Trivedi Effect® - Biofield Energy Treatment might have the impact on the intrinsic physicochemical properties of the phytoconstituents present in the ashwagandha root extract and responsible for the alteration in the relative peak height/area of treated sample compared with the control sample. As a result, the concentrations of the phytoconstituents assumed to be increased in treated sample compared with the control sample. This treated ashwagandha root extract would be helpful for designing better nutraceutical/pharmaceutical formulations which might be providing a better therapeutic response against autoimmune diseases, nervous and sexual disorders, infectious diseases, antiaging, diabetes, cancer, immunological disorders, stress, arthritis, etc.

Characterization of Physicochemical, Thermal, Structural, and Behavioral Properties of Magnesium Gluconate After Treatment with the Energy of Consciousness

Journal: International Journal of Pharmacy and Chemistry PDF  

Published: 11-Mar-17 Volume: 3 Issue: 1

DOI:10.11648/j.ijpc.20170301.11 ISSN: Not Available

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, Deborah Lea Smith, Dezi Ann Koster, Elizabeth Patric, Jagdish Singh, Kathleen Starr Vagt, Krista Joanne Callas, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Abstract

Magnesium gluconate is a potent antioxidant and widely used for the prevention and treatment of hypomagnesia. The current research was aimed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment on magnesium gluconate for the change in the physicochemical, structural, thermal and behavioral properties using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Magnesium gluconate was divided into two parts – one part was control without any Biofield Energy Treatment, while another part was treated with The Trivedi Effect® - Energy of Consciousness Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The PXRD analysis exhibited that the crystallite size of the treated sample was remarkably changed from - 24.96% to 99.98% compared with the control sample. The average crystallite size was significantly increased by 7.79% in the treated sample compared with the control sample. PSD analysis revealed that the particle sizes in The Trivedi Effect® Treated sample at d10, d50 and d90 values were decreased by 5.36%, 11.35% and 0.90%, respectively compared with the control sample. The surface area analysis revealed that surface area of the Biofield Energy Treated sample was significantly increased by 7.48% compared with the control sample. The FT-IR and UV-vis analysis showed that structure of the magnesium gluconate remained the same in both the treated and control samples. The TGA analysis revealed four steps thermal degradation of both the samples and the total weight loss of Biofield Energy Treated sample was increased by 0.12% compared with the control sample. The DSC analysis demonstrated that the melting temperature of the Biofield Energy Treated sample (171.29°C) was increased by 0.18% compared with the control sample (170.99°C). The latent heat of fusion was significantly increased by 27.09% in the treated sample compared with the control sample. The current study revealed that The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) might lead to a new polymorphic form of magnesium gluconate, which would be more soluble, bioavailable, and thermally stable compared with the untreated compound. The Biofield Treated sample could be more stable during manufacturing, delivery or storage conditions than the untreated sample. Hence, The Trivedi Effect® Treated magnesium gluconate would be very useful to design better nutraceutical and/or pharmaceutical formulations that might offer better therapeutic responses against inflammatory diseases, immunological disorders, stress, aging, and other chronic infections.

Effect of the Energy of Consciousness (The Trivedi Effect®) on the Structural Properties and Isotopic Abundance Ratio of Magnesium Gluconate Using LC-MS and NMR Spectroscopy

Journal: Advances in Biochemistry PDF  

Published: 09-Mar-17 Volume: 5 Issue: 1

DOI:10.11648/j.ab.20170501.12 ISSN: 2329-0870 (Print) 2329-0862 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, Deborah Lea Smith, Dezi Ann Koster, Elizabeth Patric, Jagdish Singh, Kathleen Starr Vagt, Krista Joanne Callas, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Abstract

Magnesium gluconate has the wide application for the prevention and treatment of hypomagnesemia. The objective of the current study was to investigate the effect of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® - Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The LC-MS analysis of both the control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.53 minutes with almost similar fragmentation pattern. The relative peak intensities of the fragment ions of the treated sample were significantly altered compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were observed almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the percentage of the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) was significantly increased in treated sample by 80.38%, compared with the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly increased compared with the control sample. Thus, the treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. The Trivedi Effect® treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects.

An Impact of Energy of Consciousness (The Trivedi Effect®) on the Physicochemical, Thermal, Structural, and Behavioral Properties of Magnesium Gluconate

Journal: Biomedical Sciences PDF  

Published: 14-Mar-17 Volume: 3 Issue: 2

DOI:10.11648/j.bs.20170302.11 ISSN: Not Available

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ariadne Esmene Afaganis, Barbara Marie Bader, Brian A. Weekes, Daphne Luisa Dumas, Denise Marie Fiedler, Dennille Mellesia Smith, Desi Pano, Donna Felice Galla, Donna Maria Alija, Elaine Barbara Mullins, Elaine M. Scorza, Ellia O'Donnell, Fabio Massimo Paciucci, Frances Goodman Warlick, Haddon Norman Salt, Inthirani Arul, Jacqueline Y. Andrews, James Jay McLeran, James Stephen Burnett, Jean Caroline White, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Abstract

Magnesium gluconate is a potent antioxidant used for the prevention and treatment of hypomagnesia. The current research was aimed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the physicochemical, structural, thermal and behavioral properties using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Magnesium gluconate was divided into two parts – one part was control, while another part was treated with The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. The PXRD analysis exhibited that the crystallite size of the treated sample was remarkably changed from range -37.52% to 180.14% compared with the control sample. The average crystallite size was reduced in the treated sample by 0.11% compared with the control sample. PSD analysis revealed that the particle sizes in the treated sample at d10 and d50 values were significantly decreased by 11.36% and 8.25%, respectively, while the particle size at d90 was increased in the treated sample by 2.43% compared with the control sample. The treated sample’s surface area was significantly increased by 9.54% compared with the control sample. The FT-IR and UV-vis analysis showed that the structure of the magnesium gluconate remained the same in both the treated and control samples. The TGA analysis revealed the four steps thermal degradation of the both samples and the total weight loss of the treated sample was reduced by 2.32% compared with the control sample. This result indicated that the thermal stability of the treated sample was improved compared with the control sample. The DSC analysis revealed that the melting temperature of the treated sample (171.05°C) was decreased by 0.17% compared with the control sample (171.34°C). The latent heat of fusion was decreased by 1.16% in the treated sample compared with the control sample. The current study infers that The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) might lead to a new polymorphic form of magnesium gluconate, which would be more soluble, bioavailable, and thermally stable compared with the untreated compound. Hence, The Trivedi Effect® Treated magnesium gluconate would be very useful to design better nutraceutical and/or pharmaceutical formulations that might offer better therapeutic responses against inflammatory diseases, immunological disorders, stress, aging and other chronic infections.

Structural Properties and Isotopic Abundance Ratio Analysis of Magnesium Gluconate Treated with the Energy of Consciousness Using LC-MS and NMR Spectroscopy

Journal: International Journal of Applied Agricultural Sciences PDF  

Published: 20-Mar-17 Volume: 3 Issue: 2

DOI:10.11648/j.ijaas.20170302.12 ISSN: 2469-7877 (Print) 2469-7885 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ariadne Esmene Afaganis, Barbara Marie Bader, Brian A. Weekes, Daphne Luisa Dumas, Denise Marie Fiedler, Dennille Mellesia Smith, Desi Pano, Donna Felice Galla, Donna Maria Alija, Elaine Barbara Mullins, Elaine M. Scorza, Ellia O'Donnell, Fabio Massimo Paciucci, Frances Goodman Warlick, Haddon Norman Salt, Inthirani Arul, Jacqueline Y. Andrews, James Jay McLeran, James Stephen Burnett, Jean Caroline White, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Abstract

The present study was aimed to evaluate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect®- Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. The LC-MS analysis of both the control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.52 min with almost similar fragmentation pattern. The relative peak intensities of the fragment ions of the treated sample were significantly altered compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) and PM+2/PM (18O/16O or 26Mg/24Mg) were significantly decreased in the treated sample by 35.97% and 66.77%, respectively compared with the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly reduced compared with the control sample. Thus, The Trivedi Effect® treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. The Trivedi Effect® Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects.